动物营养学报2016，Vol.28Issue(7)：2113-2121,9.DOI:10.3969/j.issn.1006-267x.2016.07.016
酪酸梭菌对断奶仔猪生长性能、肠道组织形态及肠道通透性的影响
Effects of Clostridium butyricum on Growth Performance, Intestinal Morphology and Intestinal Permeability of Weanling Piglets
摘要
Abstract
The purpose of this study was to evaluate the effects of Clostridium butyricum on growth perform⁃ance, intestinal morphology and intestinal permeability of weaning piglets. Eighteen ‘Duroc×Landrace×York⁃shire’ crossbred piglets with an average body weight of (5.35±1.33) kg were weaned at 21 days of age and randomly allocated into three groups:basal diet group ( control group) , basal diet+500 mg/kg Clostridium bu⁃tyricum group ( CB group) and basal diet+3 000 mg/kg zinc oxide group ( ZnO group) . Each group had six replicates, and each replicate had one piglet. The experiment lasted for 14 days. The results showed as follows:1) there were no significant differences on average daily gain and feed to gain ratio among three groups ( P>0.05) . The diarrhea rate of weaning piglets in CB group and ZnO group was significantly lower than that in control group ( P<0.05) . 2) Compared with the control group, the crypt depth of weaning piglets in CB group and ZnO group significantly decreased ( P<0.05) , and the ratio of villus height to crypt depth significantly in⁃creased ( P<0.01) . 3) On the day 14, the serum endotoxin and D⁃lactic acid concentrations of weaning piglets in CB group and ZnO group significantly decreased compared with control group ( P<0.05) , and no significant difference between CB group and ZnO group was found ( P>0.05) . 4) Compared with the control group, the mRNA expression of Occludin in ileum and colon of weaning piglets in CB group and ZnO group significantly increased ( P<0.05); compared with ZnO group, the mRNA expression of zonula occludens⁃1 ( ZO⁃1) and Occludin in ileum and colon of weaning piglets in CB group had no significant difference ( P>0.05) . The re⁃sults indicate that the dietary supplemented with Clostridium butyricum can decrease diarrhea rate and intestinal permeability, while improve intestinal mucosal morphology and tight junction protein expression. Dietary sup⁃plemented with Clostridium butyricum is comparable to the effect of the dietary supplemented with zinc oxide.关键词
断奶仔猪/酪酸梭菌/氧化锌/腹泻/肠道黏膜屏障Key words
weaning piglets/Clostridium butyricum/zinc oxide/diarrhea/intestinal mucosal barrier分类
农业科技引用本文复制引用
庞敏,卢庆萍,夏冰,张宏福..酪酸梭菌对断奶仔猪生长性能、肠道组织形态及肠道通透性的影响[J].动物营养学报,2016,28(7):2113-2121,9.基金项目
国家科技支撑项目（2012BAD39B01）；中国农业科学院创新团队 （）
