动物营养学报2016，Vol.28Issue(7)：2260-2268,9.DOI:10.3969/j.issn.1006-267x.2016.07.033
寡果糖诱导瘤胃急性酸中毒对山羊瘤胃发酵、蹄组织结构及炎症因子、金属蛋白酶表达的影响
Effects of Ruminal Acute Acidosis Induced by Fructo-Oligosaccharide on Ruminal Fermentation, Hooves Structure and Expressions of Inflammatory Cytokines and Matrix Metalloproteinase of Goats
摘要
Abstract
The present study aimed to investigate the effects of ruminal acute acidosis induced by fructo⁃oligo⁃saccharide on ruminal fermentation, hooves structure and expressions of inflammatory cytokines and matrix metalloproteinase of goats. Eight healthy crossbred goats ( Boer goat × Yangtze River delta white goats) with permanent ruminal fistulas were randomly divided into two groups, including control and test groups which was treated with fructo⁃oligosaccharide infusion (21 g/kg BW), and each group had 4 goats. Ruminal liquid and blood were collected respectively at 0 ( before infusion) , 4, 8, 12, 24 and 48 h, as well as 0, 4, 8, 24 and 48 h after infusion. The goats were slaughtered to collect for hooves tissue after 48 h of infusion. The results showed as follows, compared with control group, the average values of ruminal liquid pH, volatile fatty acids was significantly decreased in test group (P<0.05), while the average values of the concentrations of lactic acid and lipopolysaccharide ( LPS) in ruminal liquid and blood were significantly increased ( P<0.05);the re⁃sults of histology suggested that compared with control group, secondary epidermal lamellae and secondary der⁃mal lamellae were shortened in test group; the real⁃time PCR results revealed that compared with control group, the mRNA expression of tissue inhibitors of metalloproteinases⁃1 ( TIMP⁃1) was significantly decreased in test group ( P<0.05) , while those of interleukin⁃6 ( IL⁃6) , matrix metalloproteinase 2 ( MMP⁃2) and mem⁃brane type⁃1 matrix metalloproteinases ( MT1⁃MMP) in goat hooves were significantly increased ( P<0.05) . In conclusion, ruminal acute acidosis induced by fructo⁃oligosaccharide causes the disorder of ruminal fermenta⁃tion and increases concentrations of LPS and lactic acid in ruminal liquid and blood, which further changes of mRNA expressions of pro⁃inflammatory cytokines and matrix metalloproteinase genes related to laminitis, and finally induces the laminitis in goats.关键词
山羊/寡果糖/炎症因子/基质金属蛋白酶/蹄叶炎Key words
goat/fructo-oligosaccharide/inflammatory cytokines/matrix metalloproteinase/laminitis分类
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冯泮飞,刘军花,叶慧敏,朱伟云,毛胜勇..寡果糖诱导瘤胃急性酸中毒对山羊瘤胃发酵、蹄组织结构及炎症因子、金属蛋白酶表达的影响[J].动物营养学报,2016,28(7):2260-2268,9.基金项目
国家自然科学基金 （）
