南方医科大学学报2016，Vol.36Issue(7)：898-903,6.DOI:10.3969/j.issn.1673-4254.2016.07.04
埃兹蛋白477位酪氨酸的磷酸化在神经生长因子前体促进乳腺癌细胞侵袭中起关键作用
Phosphorylation of ezrin Tyr477 is critical in invasion of breast cancer cells stimulated by precursor of nerve growth factor
摘要
Abstract
Objective To investigate the effect of precursor of nerve growth factor (proNGF) in promoting invasion of breast cancer cells and its relation with ezrin expression and phosphorylation of ezrin Thr567 and Tyr477. Methods Human breast cancer cell lines MDA-MB-231 and MCF-7 were stimulated by gradient concentrations of proNGF (0, 2.5, 5 and 10 ng/mL) for 16 h, and the invasion of the cells was assessed with Transwell assay. The expression of ezrin and the phosphorylation of ezrin Thr567 and ezrin Tyr477 in the treated cells were examined by Western blotting. MDA-MB-231 cells were transfected with pEnter-His-ezrinY477F (a dominant negative mutant) to study the role of phosphrylation of ezrin Tyr477 in the invasion of breast cancer cell stimulated by proNGF. Results proNGF significantly promoted MDA-MB-231 and MCF-7 cell invasion in a concentration-dependent manner (P<0.05), and concentration- and time-dependently increased the phosphorylation of ezrin Tyr477 (P<0.05) without affecting the expression of ezrin or the phosphorylation of ezrin Thr567. The specific inhibitor of src, SKI-606, significantly inhibited the phosphorylation of ezrin Tyr477 induced by proNGF. Transfection with pEnter-His-ezrinY477F inhibited proNGF-induced invasion and phosphorylation of ezrin Tyr477 in MDA-MB-231 cells (P<0.05). Conclusion Phosphorylation of ezrin Tyr477 plays a critical role in the invasion of breast cancer cells stimulated by proNGF via proNGF/src/ezrin Tyr477 pathway.关键词
乳腺癌/神经生长因子前体/埃兹蛋白/侵袭Key words
breast cancer/precursor of nerve growth factor/ezrin/invasion引用本文复制引用
李福喜,邵文凤,唐芮,于晓然,温桥生,余焱林,熊静波..埃兹蛋白477位酪氨酸的磷酸化在神经生长因子前体促进乳腺癌细胞侵袭中起关键作用[J].南方医科大学学报,2016,36(7):898-903,6.基金项目
国家自然科学基金（81071810，81172058）Supported by National Natural Science Foundation of China (81071810，81172058) （81071810，81172058）
