南方医科大学学报2016，Vol.36Issue(7)：904-908,5.DOI:10.3969/j.issn.1673-4254.2016.07.05
ApoE4在U87细胞中可增加GSK-3β的表达及Tau的磷酸化
ApoE4 increases glycogen synthase kinase 3βexpression and Tau phosphorylation in U87 cells
摘要
Abstract
Objective To explore the relations among apolipoprotein E4, Tau protein and glycogen synthase kinase 3β(GSK-3β). Methods U87 cells were transfected with pIRES-EGFP (control) or the recombinant plasmids ApoE4/pIRES-EGFP or ApoE3/pIRES-EGFP, and the expression levels of p-Tau/Tau and GSK-3βin the cells were examined with Western blotting. To further confirm the effect of ApoE on GSK-3βand p-Tau expressions, a short interfering RNA (siRNA) targeting ApoE (ApoE-siRNA) was transfected into U87 cells via Lipofectamine 2000 and the protein expressions were examined 24 h later. Results Compared with those in the control group, the expressions levels of both GSK-3β and p-Tau/Tau increased significantly in the cells transfected with ApoE4 and ApoE3 plasmids (P<0.01), and the ApoE4 plasmid produced a more potent effect than the ApoE3 plasmid on the protein expressions (P<0.01). ApoE knockdown resulted in significantly reduced expressions of GSK-3β (P<0.001) and p-Tau (P<0.01) in the cells. Conclusion ApoE4 can enhance Tau phosphorylation though upregulating GSK-3β, which sheds light on a new role of ApoE4 in Alzheimer's disease.关键词
载脂蛋白E/阿尔茨海默病(AD)/Tau蛋白/糖原合酶激酶3βKey words
ApoE/Alzheimer's disease/Tau/glycogen synthase kinase 3β引用本文复制引用
何颜结,卫佩如,伍巧燕,张馨宇,张兴梅,刘晓加,王方..ApoE4在U87细胞中可增加GSK-3β的表达及Tau的磷酸化[J].南方医科大学学报,2016,36(7):904-908,5.基金项目
国家自然科学基金（81471388）；广东省自然科学基金（2015A030313235）；广州市科技计划项目（2014J4100148）；教育部大学生创新创业训练计划项目（201312121020）Supported by National Natural Science Foundation of China (81471388) （81471388）
