南方医科大学学报2016，Vol.36Issue(7)：909-914,6.DOI:10.3969/j.issn.1673-4254.2016.07.06
子宫内膜巨噬细胞通过调控血管内皮生长因子A的表达影响胚胎着床
Uterine macrophages affect embryo implantation via regulating vascular endothelial growth factor A in mice
摘要
Abstract
Objective To investigate the role of endometrial macrophages in embryo implantation and in regulating the expression of vascular endothelial growth factor A (VEGFA) in mouse endometrium during the peri-implantation period. Method At D3.5 (D0.5 defined as the morning when a vaginal plug was observed), pregnant mice were divided randomly into experimental group, control group and blank group. In the experimental group, the mice were subjected to intrauterine injection of clodronate liposomes on the left side of uterus to eliminate the macrophages, and PBS liposomes on the right side. PBS liposomes and PBS were administered in the control and blank groups, respectively. The uterine tissues were collected on D5.5 and stained with trypan blue to show the implantation sites. Flow cytometry was performed to examine the percentage of F4/80+ CD11b+ macrophages macrophages in the uterus. F4/80+ macrophage population within the endometrium and ovary and changes in VEGFA expression at the implantation and non-implantation sites were examined using immunohistochemistry. Results Endometrial F4/80 + CD11b + macrophages macrophages were significantly reduced by 74%following intrauterine injection of clodronate liposomes (P<0.05). The number of macrophages in the ovaries showed no significant difference among the 3 groups. In the experimental group, the left side of the uterine showed imcomplete cavity closure with a lower number of implantation site than the right side (2.20±1.81 vs 5.10±1.91, P<0.05). VEGFA expression at the implantation site were significantly decreased in the endometrium on the left side with macrophage suppression as compared with that on the right side (P<0.05). Conclusion Endometrial macrophages appear to modulate uterine receptivity by regulating the expression of VEGFA to affect embryo implantation, suggesting the important role of macrophages in embryo implantation.关键词
子宫内膜巨噬细胞/氯膦酸二钠的脂质体/宫腔内注射/胚胎着床/血管内皮生长因子AKey words
Uterine macrophages/clodronate liposomes/intrauterine injection/embryo implantation/vascular endothelial growth factor A引用本文复制引用
王京,谢多,刘勉,龚艺,史潇,韦锦燕,全松..子宫内膜巨噬细胞通过调控血管内皮生长因子A的表达影响胚胎着床[J].南方医科大学学报,2016,36(7):909-914,6.基金项目
国家自然科学基金（81170575）Supported by National Natural Science Foundation of China (81170575) （81170575）
