南方医科大学学报2016，Vol.36Issue(7)：921-926,6.DOI:10.3969/j.issn.1673-4254.2016.07.08
远华蟾蜍精通过调节应激与凋亡通路诱导结直肠癌细胞的凋亡
Mechanism of colon cancer cell apoptosis induced by telocinobufagin：role of oxidative stress and apoptosis pathway
摘要
Abstract
Objective To investigate the effects of telocinobufagin on viability and apoptosis of colorectal cancer (CRC) cells and explore the mechanism of telocinobufagin-induced apoptosis. Methods MTT assay was performed to detect the viability of CRC cells exposed to telocinobufagin. Nuclear staining with Hoechst 33342 and flow cytometry were used to analyze the cell death of CRC cells. Expressions of proteins related with cell apoptosis and oxidative stress were determined with Western blotting. Resuts Telocinobufagin decreased the viability of CRC cells in a time-and dose-dependent manner. The presence of karyopycnosis and apoptotic bodies together with the results of flow cytometry suggested that telocinobufagin induced cell apoptosis to cause cell death. Western blotting showed that telocinobufagin exposure of the cells resulted in upregulated p53 and Bax protein expressions and promoted cleavage of caspase 9 and PARP. Telocinobufagin induced phosphorylation of Bad and PARP cleavage, and suppressed phosphorylation of IΚBαand TAK1 and expression of survivin in the cells. Conclusion Telocinobufagin can decrease the viability of CRC cells by inducing cell apoptosis, which involves p53-mediated Bax activation and inhibition of the IAP pathway.关键词
远华蟾蜍精/结直肠癌/凋亡/抗体芯片Key words
telocinobufagin/colorectal cancer/apoptosis/antibody array引用本文复制引用
梁顺添,李源,李晓武,王俊江,谭福仙,韩钦芮,李璐,姚学清,孙学刚..远华蟾蜍精通过调节应激与凋亡通路诱导结直肠癌细胞的凋亡[J].南方医科大学学报,2016,36(7):921-926,6.基金项目
广东省社会发展领域科技计划项目（2014A020212636）；2015年广东大学生科技创新培育专项资金（“攀登计划”专项资金）；南方医科大学2015年度国家级、省级、校级大学生创新创业训练计划项目 （）
