南方医科大学学报2016，Vol.36Issue(7)：936-940,5.DOI:10.3969/j.issn.1673-4254.2016.07.11
前列腺素E2对大鼠巨噬细胞株NR8383合成血管内皮生长因子促进人脐静脉血管内皮细胞成管、迁移的影响
Media of rat macrophage NR8383 cells with prostaglandins E2-induced VEGF over-expression promotes migration and tube formation of human umbilical vein endothelial cells
摘要
Abstract
Objective To investigate the effect of prostaglandins E2 (PGE2) in enhancing vascular endothelial growth factor (VEGF) expression in a rat macrophage cell line and the effect of the media from PGE2-inuced rat macrophages on angiogenetic ability of human umbilical vein endothelial cells (HUVECs) in vitro. Methods Western blotting and qPCR were employed to investigate the expressions of VEGF protein and mRNAs in rat macrophage cell line NR8383 stimulated by PGE2 in the presence or absence of EP2 receptor inhibitor (AH6809) and EP4 receptor inhibitor (AH23848). Conditioned supernatants were obtained from different NR8383 subsets to stimulate HUVECs, and the tube formation ability and migration of the HUVECs were assessed with Transwell assay. Results PGE2 stimulation significantly enhanced the expression of VEGF protein and mRNAs in NR8383 cells in a dose-dependent manner. The supernatants from NR8383 cells stimulated by PGE2 significantly enhanced tube formation ability of HUVECs (P<0.05) and promoted the cell migration. Such effects of PGE2 were blocked by the application of AH6809 and AH23848. Conclusion PGE2 can dose-dependently increase VEGF expression in NR8383 cells, and the supernatants derived from PGE2-stimulated NR8383 cells can induce HUVEC migration and accelerate the growth of tube like structures. PGE2 are essential to corpus luteum formation by stimulating macrophages to induce angiogenesis through EP2/EP4.关键词
前列腺素E2/大鼠肺泡巨噬细胞株NR8383/人脐静脉血管内皮细胞/血管内皮生长因子Key words
prostaglandin E2/rat pulmonary alveolar macrophage NR8383/human umbilical vein endothelial cell/vascular endothelial growth factor引用本文复制引用
刘勉,龚艺,韦锦燕,谢多,王京,余艳红,全松..前列腺素E2对大鼠巨噬细胞株NR8383合成血管内皮生长因子促进人脐静脉血管内皮细胞成管、迁移的影响[J].南方医科大学学报,2016,36(7):936-940,5.基金项目
国家自然科学基金（81170575）Supported by National Natural Science Foundation of China (81170575) （81170575）
