南方医科大学学报2016，Vol.36Issue(7)：990-996,7.DOI:10.3969/j.issn.1673-4254.2016.07.21
miR-101对结直肠癌细胞SW620生物学特性的影响
Effect of miR-101 on biological characteristics of colorectal cancer cell line SW620
摘要
Abstract
Objective To investigate the effect of miR-101 on biological behaviors of colorectal cancer cell line SW620. Methods CCK-8 method, colony formation assay, cell cycle analysis and apoptosis analysis were applied to assess the effects of miR-101 on cell proliferation, invasion and apoptosis of SW620 cells. Results Over-expression of miR-101 in SW620 cells significantly suppressed the cell proliferation and attenuated the colony-forming ability of the cells. Flow cytometry showed that over-ex-pression of miR-101 in SW620 cells caused obvious cell cycle arrest in G2/M and G1 phases, and significantly increased the cell apoptosis rate. Conclusion Over-expression of miR-101 can inhibit the proliferation, cause cell cycle arrest and promote apop-tosis of colorectal cancer SW620 cells.关键词
结直肠癌/miR-101/SW620/增殖/凋亡/细胞周期/生物学特性Key words
colorectal cancer cells/miR-101/SW620 cells/proliferation/apoptosis/cell cycle引用本文复制引用
刘燕,陆滟霞,周敏,郑林,李学农..miR-101对结直肠癌细胞SW620生物学特性的影响[J].南方医科大学学报,2016,36(7):990-996,7.基金项目
国家自然科学基金（81272758，81302158）Supported by National Natural Science Foundation of China (81272758,81302158) （81272758,81302158）
