南方医科大学学报2016，Vol.36Issue(7)：1008-1013,6.DOI:10.3969/j.issn.1673-4254.2016.07.24
组织工程血管培养生物反应器出口端加载阻力的应力刺激形成系统的设计与验证
Design and verification of Luo-Ye pump-based stress formation for cultivation of tissue-engineered blood vessel
摘要
Abstract
Objective To improve Luo-Ye pump-based stress-forming system and optimize the stimulating effect on smooth muscle cells during cultivation of tissue-engineered blood vessels (TEBV). Methods A new Luo-Ye pump-based TEBV 3D culture system was developed by adding an air pump to the output of the bioreactor. A pressure guide wire was used to measure the stress at different points of the silicone tube inside the TEBV bio-reactor, and fitting curves of the stress changes over time was created using Origin 8.0 software. The TEBVs were constructed by seeding vascular smooth muscle cells (VSMCs) isolated from human umbilical artery on polyglycolic acid (PGA) and cultured under dynamic conditions with 40 mmHg resistance (improved group), dynamic conditions without resistance (control group) or static condition (static group) for 4 weeks. The harvested TEBVs were then examined with HE staining, masson staining, α-SMA immunohistochemical staining, and scanning and transmission electron microscopy with semi-quantitative analysis of collagen content andα-SMA expression. Results The measured stress values and the fitting curves showed that the stress stimuli from the Luo-Ye pump were enhanced by adding an air pump to the output of the bioreactor. Histological analysis revealed improved VSMC density, collagen content andα-SMA expression in the TEBVs constructed with the improved method as compared with those in the control and static groups. Conclusion Adding an air pump to the Luo-Ye pump significantly enhances the stress stimulation in the TEBV 3-D culture system to promote the secretion function of VSMCs.关键词
组织工程血管/罗叶泵/应力/三维培养/平滑肌细胞Key words
tissue-engineered blood vessels/Luo-Ye pump/stress/three-dimensional culture/vascular smooth muscle cell引用本文复制引用
廖文君,陈婉雯,文章,吴岳恒,李东风,周嘉辉,郑坚奕,林展翼..组织工程血管培养生物反应器出口端加载阻力的应力刺激形成系统的设计与验证[J].南方医科大学学报,2016,36(7):1008-1013,6.基金项目
广东省科技计划项目 （）
