森林与环境学报2016，Vol.36Issue(3)：306-311,6.DOI:10.13324/j.cnki.jfcf.2016.03.009
雷公藤愈伤组织的生长特征及内酯醇的积累
Growth traits and triptolide accumulation of Tripterygium wilfordii callus
摘要
Abstract
This study investigated the effects of different explants, culture media and pH on induction of Tripterygium wilfordii callus. And then physiological and biochemical properties and triptolide accumulation in the callus were further analyzed. The results indicated that, to induce T. wilfordii callus, the best explants were tender leaves of tissue culture seedlings, the most suitable medium was MS, and the optimal pH values ranged from 5.8 to 6.1. The activities of SOD and POD both reached the maximum at the middle stage (4th week) and the end (8th week) of the callus growth period, when MDA content and PAL activity showed relatively low values. At the stationary growth phase, SOD and POD had low activities, while MDA content and PAL activity were high. As to triptolide content in the callus of different explants, the root callus and the leaf callus shared similar levels, significantly higher than the stem callus. The callus produced higher triptolide amount at the latter stage ( 6th to 8th week) of the growth period.关键词
雷公藤/愈伤组织/生理生化特征/雷公藤内酯醇Key words
Tripterygium wilfordii Hook. F/callus/physiological and biochemical properties/triptolide分类
农业科技引用本文复制引用
孙睿,朱留刚,封磊,宋萍,洪伟,吴承祯..雷公藤愈伤组织的生长特征及内酯醇的积累[J].森林与环境学报,2016,36(3):306-311,6.基金项目
国家自然科学基金项目(31000264,31470577)；福建省高校产学合作科技重大项目(2014N5005)；福建省高等学校新世纪优秀人才支持计划(JA12089)；福建农林大学林学院青年科学基金(6112C039U)。 （31000264,31470577）
