福建农林大学学报（自然科学版）2016，Vol.45Issue(4)：420-426,7.DOI:10.13323/j.cnki.j.fafu(nat.sci.).2016.04.009
莆田黑鸭MHCⅠ基因外显子2的遗传多态性
Genetic polymorphism of exon 2 in MHCⅠ gene of Putian black duck
摘要
Abstract
To establish theoretical basis for duck breeding of disease-resistance species, genetic polymorphism analysis was conducted by PCR amplification and DNA sequencing of exon 2 in MHCⅠ gene from Putian black duck ( hereafter referred to as exon2) . Results showed that exon 2 had high degree of genetic polymorphism, whose nucleotide variance was mainly attributed to transversion, and higher rate of synonymous substitution than non-synonymous substitution. The total content of GC in exon 2 was 59%, with the highest portion coming from the 3rd codon ( 82. 7%) . Amino acid composition analysis illustrated that exon 2 had high level of hydrophobicity, which might play a significant role in maintaining the structure and function of MHC. Moreover, cluster analysis showed that Putian black duck had a closer relationship with goose, and farther with chicken.关键词
莆田黑鸭/主要组织相容性复合体Ⅰ( MHCⅠ)/DNA测序/遗传多态性Key words
Putian black duck/major histocompatibility complex Ⅰ( MHCⅠ)/DNA sequencing/genetic diversity分类
农业科技引用本文复制引用
汪稳,林威敏,陈静,胡宇平,毛小鸿,肖天放..莆田黑鸭MHCⅠ基因外显子2的遗传多态性[J].福建农林大学学报（自然科学版）,2016,45(4):420-426,7.基金项目
福建省高等学校科技项目(JA09082)；福建省高校产学合作科技重大项目(2012N5001) （JA09082）
