口腔疾病防治2016，Vol.24Issue(8)：459-463,5.DOI:10.12016/j.issn.2096-1456.2016.08.005
Wnt通路抑制剂XAV-939对牙髓干细胞成脂分化的影响
Wnt inhibitor XAV-939 promote the adipogenic differentiation of dental pulp stem cells
摘要
Abstract
Objective To evaluate the effect of Wnt inhibitor XAV⁃939 on the proliferation and adipogenic differen⁃tiation of dental pulp stem cells (DPSCs). Methods The effect of XAV⁃939 on the proliferation of DPSCs was analyzed by cell counting kit⁃8(CCK⁃8). The effect of XAV⁃939 on the adipogenic differentiation of DPSCs was analyzed by oil red staining. Quantificational real⁃time polymerase chain reaction (qRT⁃PCR) was used to analyze the gene expression of glycogen synthase kinase⁃3β(GSK3β) andβ⁃catenin which were Wnt signaling marker genes, and peroxisome prolif⁃erator activated receptor⁃γ2 (PPARγ2) and CCAAT/enhancer binding proteinα(C/EBPα) which were adipogenic mark⁃er genes. Results After 14 days, XAV⁃939 up⁃regulates expression of GSK3β、PPARγ2 and C/EBPα, while down⁃reg⁃ulatesβ⁃catenin. Conclusion XAV⁃939 could promote the adipogenic differentiation of DPSCs by inhibiting Wnt sig⁃nalling pathway.关键词
牙髓干细胞/成脂分化/Wnt通路/XAV-939/细胞增殖Key words
Dental pulp stem cells/Adipogenic differentiation/Wnt passway/XAV-939/Cell proliferation分类
医药卫生引用本文复制引用
文军,徐帅妹,刘影..Wnt通路抑制剂XAV-939对牙髓干细胞成脂分化的影响[J].口腔疾病防治,2016,24(8):459-463,5.基金项目
广东省自然科学基金 （）
