口腔疾病防治2016，Vol.24Issue(8)：464-468,5.DOI:10.12016/j.issn.2096-1456.2016.08.006
汉防己甲素抑制口腔癌细胞增殖与β-catenin表达的研究
Study of the cellular growth inhibited by tetrandrine with the expression of β-catenin in oral squamous cell carcinoma cell line
摘要
Abstract
Objective To study the effect of tetrandrine (TET) on oral squamous cell carcinoma cell line Tca8113 and its influence against the expression ofβ⁃catenin. Methods CCK⁃8 method was used to detect the effect of TET on Tca8113 by different dose and time. Western blot assay was used to detect the expression of β⁃catenin after treatment by TET meanwhile the distribution of the β⁃catenin treated by TET was detected by the immunofluorescence method. Results With the increasing dose of TET, the inhibition rate against Tca8113 was raising (P < 0.05). The half maxi⁃mal inhibitory concentration (IC50) value of TET against Tca8113 was 64μmol/L. With this concentration, the inhibition rate against Tca8113 exhibited a time⁃dependent manner. The results of western blot revealed that the expression of β⁃catenin was significantly inhibited after treatment by TET (P < 0.05). The results of immunofluorescence method showed that the β⁃catenin protein mainly located at nucleus in Tca8113, while after treatment by TET, the β⁃catenin protein mainly located at cellular membrane and cytoplasm in a diffused distribution manner. Conclusion Our data suggest that TET can inhibit the cell growth of oral squamous cell carcinoma, and influence the cellular distribution ofβ⁃catenin protein in tumor cells.关键词
汉防己甲素/口腔癌/β-连环蛋白/增殖/细胞培养Key words
Tetrandrine/Oral squamous cell carcinoma/β-catenin/Cell growth/Cell culture分类
临床医学引用本文复制引用
程燕飞,钟永荣..汉防己甲素抑制口腔癌细胞增殖与β-catenin表达的研究[J].口腔疾病防治,2016,24(8):464-468,5.基金项目
广东省中医药管理局基金 （）
