广东医学2016，Vol.37Issue(12)：1761-1763,3.
右美托咪定对创伤性脑损伤大鼠认知功能和P2 X7表达的影响
Effects of dexmedetomidine on cognitive function and hippocampus P2X7 expression in rats with traumatic brain injury
摘要
Abstract
Objective To observe the effects of Dexmedetomidine ( Dex) on cognitive function and neuroinflam-mation after traumatic brain injury (TBI), and to discuss the possible mechanism of P2X7 receptor in these effects. Methods Eighty Male SD rats (3~4 months old) were randomly divided into control group , Dex group, TBI group, and TBI+Dex group (n=20).The TBI model was established.Intraperitoneal Dex (20μg· kg-1) was given to the subjects in Dex group and TBI+Dex group , while saline was given in control group and TBI group .Moris water maze test was per-formed to evaluate the cognitive function 6 days after TBI .ELISA was used to quantify the concentration of interleukin -1β(IL-1β) in hippocampus.Western blot was used to measure the expression of P 2X7 in hippocampus.Results Compared with control group , the escape latency was significantly prolonged , with the time of staying at the original plat-form quadrant shortened, between Day 3 and 5 in TBI group (P<0.05);meanwhile, the concentration of IL -1β, and the expression of P2X7 in hippocampus were significantly increased in TBI group ( P <0.001).Compared with TBI group, the escape latency was significantly shortened , with the time of staying at the original platform quadrant prolonged in TBI+Dex group (P<0.05); meanwhile, the concentration of IL -1β, and the expression of P2X7 in hippocampus were significantly reduced in TBI +Dex group (P<0.001).Conclusion Dexmedetomidine can attenuate TBI -induced cognitive decline and neuroinflammation via inhibiting the activity of P 2X7 receptor.关键词
创伤性脑损伤/神经炎症/认知功能障碍/右美托咪定/P2X7Key words
traumatic brain injury/neuroinflammation/cognitive dysfunction/dexmedetomidine/P2X7引用本文复制引用
郑彬,张顺才,曾彦茹,李平,付晓东,阮祥才,许立新,佘守章..右美托咪定对创伤性脑损伤大鼠认知功能和P2 X7表达的影响[J].广东医学,2016,37(12):1761-1763,3.基金项目
广东省医学科研基金资助项目 （）
