广东医学2016，Vol.37Issue(14)：2065-2068,4.
三突变型低氧诱导因子－1α基因对老年小鼠缺血下肢血管的新生效应
Angiogenic effects of the HIF-1αwith triple-point mutations in hind-limb ischemia in aged mice
摘要
Abstract
Objective To explore the angiogenic effects of the HIF -1αwith triple-point mutations in hind -limb ischemia in aged mice .Methods Twenty-four aged mice under unilateral artery ligation were randomly divided into 3 test groups, in which normal saline, adenovirus (Ad) vector particles containing the gene for LacZ (Ad-LacZ), and HIF-1a 402/564/803 (triple-point mutant, Ad-TM) were intramuscularly injected into the ischemia hind limb , respectively .Serial contrast-enhanced ultrasonic perfusion imaging of the proximal adductor muscles in the ischemia and contralateral non-ischemia hind limbs was performed .On Day 28 postoperatively , the muscle specimens were harvested and the density of the capillaries was determined by immunofluorescence analysis using antibodies against Bandeira simpli -ficifolia-1 lectin ( BS-1 lectin) .Results Immediately after femoral artery ligation , blood flow ( as a percentage of that in the non-ischemia hind limb) was reduced to 15%in all groups (P>0.05).Although blood flow gradually recovered over time in all groups , recovery was persistently better in the Ad -TM group as compared with the other groups .On Day 28, the blood flow ratio was significantly increased in the Ad -TM group [(66.6 ±8.9)%] as compared with NS group [(34.2 ±6.3)%] and Ad-LacZ group [(35.7 ±5.4)%] (P<0.01).Capillaries density (expressed as capillary/muscle fiber) in Ad-TM group was 1.06 ±0.10, significantly superior to those in NS group (0.36 ±0.04) and Ad-LacZ group (0.35 ±0.04) (P<0.01).Conclusion The angiogenic effects were enhanced in aged mice hind -limb is-chemia by using HIF-1αwith triple-point mutations .关键词
低氧诱导因子-1α/对比超声/下肢缺血/血管新生Key words
hypoxia inducible factor -1α/contrast enhanced ultrasound/hind-limb ischemia/angiogensis引用本文复制引用
谢佳佳,李瑜辉,唐其东,吴平生,宾建平..三突变型低氧诱导因子－1α基因对老年小鼠缺血下肢血管的新生效应[J].广东医学,2016,37(14):2065-2068,4.基金项目
国家自然科学基金资助项目编号:81401426 （）
