甘肃农业科技Issue(6):21-22,23,3.DOI:10.3969/j.issn.1001-1463.2016.06.008
大蒜微型鳞茎诱导培养基的筛选初报
A Preliminary Report on Screen the Induction Medium of Garlic Micro Bulb
摘要
Abstract
The Jimsar white garlic as experimental materials, using garlic bulb as explants, Initial culture is carried out to obtain green bud in B5+6-BA 0.5 mg/ L +NAA0.5 mg/L + sucrose 30 g/L + agar 4.5 g/L medium, on the basis of this study, the formula of the culture medium for inducing the micro bulb in vitro is developed. The result shows that 1/2 MS+IBA 1.5 mg/L+NAA 0.1 gm/L 1/2 MS + IBA 1.5 mg/L + sugar + 50 g/L + agar 4.5 g/L is a better culture medium formula for inducing bulb in tissue culture plantlet, the garlic green shoots began forming a bulb the in 45th day, the bulb diameter up to 0.3~0.5 cm in 90th day under the culture medium.关键词
组织培养/鳞茎盘/诱导, 培养基/大蒜Key words
Tissue culture/Bulb plate/Induction/Culture medium/Garlic分类
农业科技引用本文复制引用
王云,蒲建刚,王德贤,常德昌,王琰,缑建民,葛亮..大蒜微型鳞茎诱导培养基的筛选初报[J].甘肃农业科技,2016,(6):21-22,23,3.基金项目
甘肃省科技支撑计划项目“陇东南大蒜脱毒快繁技术研究及示范”(1204NKCE105)部分内容 ()