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大蒜微型鳞茎诱导培养基的筛选初报

王云 蒲建刚 王德贤 常德昌 王琰 缑建民 葛亮

甘肃农业科技Issue(6):21-22,23,3.
甘肃农业科技Issue(6):21-22,23,3.DOI:10.3969/j.issn.1001-1463.2016.06.008

大蒜微型鳞茎诱导培养基的筛选初报

A Preliminary Report on Screen the Induction Medium of Garlic Micro Bulb

王云 1蒲建刚 1王德贤 1常德昌 2王琰 1缑建民 1葛亮1

作者信息

  • 1. 甘肃省天水市农业科学研究所,甘肃 天水 741001
  • 2. 甘肃省天水市果树研究所,甘肃 天水741002
  • 折叠

摘要

Abstract

The Jimsar white garlic as experimental materials, using garlic bulb as explants, Initial culture is carried out to obtain green bud in B5+6-BA 0.5 mg/ L +NAA0.5 mg/L + sucrose 30 g/L + agar 4.5 g/L medium, on the basis of this study, the formula of the culture medium for inducing the micro bulb in vitro is developed. The result shows that 1/2 MS+IBA 1.5 mg/L+NAA 0.1 gm/L 1/2 MS + IBA 1.5 mg/L + sugar + 50 g/L + agar 4.5 g/L is a better culture medium formula for inducing bulb in tissue culture plantlet, the garlic green shoots began forming a bulb the in 45th day, the bulb diameter up to 0.3~0.5 cm in 90th day under the culture medium.

关键词

组织培养/鳞茎盘/诱导, 培养基/大蒜

Key words

Tissue culture/Bulb plate/Induction/Culture medium/Garlic

分类

农业科技

引用本文复制引用

王云,蒲建刚,王德贤,常德昌,王琰,缑建民,葛亮..大蒜微型鳞茎诱导培养基的筛选初报[J].甘肃农业科技,2016,(6):21-22,23,3.

基金项目

甘肃省科技支撑计划项目“陇东南大蒜脱毒快繁技术研究及示范”(1204NKCE105)部分内容 ()

甘肃农业科技

1001-1463

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