中国妇幼健康研究2016，Vol.27Issue(7)：816-818,830,4.DOI:10.3969/j.issn.1673-5293.2016.07.010
人乳头瘤病毒 E6／E7 mRNA检测在宫颈癌筛查中的意义
Significance of human papillomavirus E6/E7mRNA detection in cervical cancer screening
摘要
Abstract
Objective To evaluate the significance of human papillomavirus (HPV) E6/E7mRNA detection in cervical cancer screening . Methods A total of 3 381 women who had taken an opportunistic cervical cancer screening received thin -prep cytologic test (TCT), HPV DNA and HPV E6/E7mRNA examinations.Among them, 371 cases with suspicious results underwent colposcopy guided biopsy .The results of their TCT , HPV DNA and HPVE6/E7mRNA tests and the pathologic results were recorded and analyzed by SPSS 19.0 software. Results The total positive rate of the HPV E6/E7mRNA test was 16.47% (557/3 381).The positive rate of HPV E6/E7mRNA in patients with inflammation and cervical intraepithelial neoplasia Ⅰ(CIN Ⅰ) was 60.73% (116/191) and 68.60% (83/121), respectively.The difference was not significant (F=1.98, P>0.05).The mRNA expression levels of patients with inflammation and patients with CIN Ⅰwas 7 781.26 ±2 686.13 and 7 498.47 ±2 860.08 copy/mL, respectively.The difference was also not significant (t=0.07, P>0.05).However, the positive rate and mRNA expression levels of CIN Ⅱ-Ⅲpatients (89.10%[49/55];30 716.66 ± 10 281.89 copy/mL) were significantly higher than those of patients with inflammation (χ2 =15.55, t=2.19) and CINⅠpatients (χ2 =8.47, t=2.18).The differences were all statistically significant (all P<0.05).Only HPV E6/E7mRNA test results were significantly different (χ2 =15.45,P<0.05) in patients with different biopsy grade (≤CINⅠor≥CINⅡ).HPV E6/E7mRNA showed its highest sensitivity (89.83%) while TCT had the highest specificity (62.18%) in the diagnosis of CIN Ⅱand above.The optimal operating point of HPV E6/E7mRNA was 1 637.48copy/mL.The sensitivity in diagnosing high degree of CIN lesions was 79.70%and the specificity was 63.50%.The area under ROC curve was 0.75 (P<0.05).Conclusion HPV E6/E7mRNA test can detect and diagnose CIN lesions with relatively high sensitivity and specificity .Thus, it has important significance in cervical cancer screening .关键词
人乳头瘤病毒/E6/E7mRNA/宫颈上皮内瘤变/宫颈癌筛查Key words
human papilloma virus (HPV)/E6/E7mRNA/cervical intraepithelial neoplasia (CIN)/cervical cancer screening分类
医药卫生引用本文复制引用
张永欣,张璐..人乳头瘤病毒 E6／E7 mRNA检测在宫颈癌筛查中的意义[J].中国妇幼健康研究,2016,27(7):816-818,830,4.基金项目
烟台市科技计划资助项目 （）
