广西医学2016，Vol.38Issue(7)：901-905,5.DOI:10.11675/j.issn.0253-4304.2016.07.02
肿瘤坏死因子-α对人脐静脉内皮细胞中组织蛋白酶K表达的影响
Effect of tumor necrosis factor-αon cathepsin K expression in human umbilical vein endothelial cells
张良 1张翼 1唐秋红 2林楚伟 3周胜华4
作者信息
- 1. 湖南省人民医院心内科，长沙市 410005
- 2. 中南大学湘雅二医院肾内科，长沙市 410011
- 3. 长沙市中心医院心内科，长沙市410004
- 4. 中南大学湘雅二医院心内科，长沙市 410011
- 折叠
摘要
Abstract
Objective To observe the effect of tumor necrosis factor-α( TNF-α) with different concentrations and different actuation durations on the expression of cathepsin K (Cat K) in human umbilical vein endothelial cells (HUVECs).Methods HUVECs in logarithmic growth phase were used in the experiment .The HUVECs were divided into normal control group ,0.1 ng/ml TNF-αgroup,1 ng/ml TNF-αgroup,10 ng/ml TNF-αgroup and 100 ng/ml TNF-αgroup treated with DMEM culture medium,0.1 ng/ml TNF-α,1ng/ml TNF-α,10 ng/ml TNF-αand 100 ng/m for 24 hours respectively .Other HUVECs were divided into control group ,TNF-α6 h group,TNF-α12 h group, TNF-α24 h group and TNF-α48 h group.The control group was treated with DMEM culture medium for 24 hours,and the other groups were treated with 10 ng/ml TNF-αfor the corresponding time .The expressions of Cat K mRNA and protein were detected in HUVECs of each group .Results The expression levels of Cat K mRNA and protein in the groups with different TNF-αconcentrations increased compared to the normal control group(P<0.05).The expression levels of Cat K mRNA and protein increased in the order of 0.1 ng/ml TNF-αgroup,1 ng/ml TNF-αgroup and 10 ng/ml TNF-αgroup(P<0.05).The expression levels of Cat K mRNA and protein in 100 ng/ml TNF-αgroup were lower than those in 10 ng/ml TNF-αgroup(P<0.05).Compared to the control group,the expression levels of Cat K mRNA and protein in the groups treated with TNF-αfor different times increased(P<0.05).The expression levels of Cat K mRNA and protein increased in the order of TNF-α6 h group,TNF-α12 h group and TNF-α24 h group(P<0.05).The expression levels of Cat K mRNA and protein in TNF-α48 h group were significantly lower than those in TNF-α24 h group(P<0.05).Conclusion Within certain action concentration and actuation duration , TNF-αcan stimulate the expression of Cat K in HUVEC in a time-and dose-dependent manner. TNF-αmight promote the occurrence of arteriosclerosis by promoting Cat K expression .关键词
组织蛋白酶K/人脐静脉内皮细胞/肿瘤坏死因子-α/动脉硬化Key words
Cathepsin K/Human umbilical vein endothelial cells/Tumor necrosis factor-α/Arteriosclerosis分类
临床医学引用本文复制引用
张良,张翼,唐秋红,林楚伟,周胜华..肿瘤坏死因子-α对人脐静脉内皮细胞中组织蛋白酶K表达的影响[J].广西医学,2016,38(7):901-905,5.
