华北农学报2016，Vol.31Issue(3)：44-50,7.DOI:10.7668/hbnxb.2016.03.007
利用 DDRT-PCR 鉴定芸芥自交不亲和系与自交亲和系的差异表达 cDNA
Identification of cDNA Fragments in Self-incompatible and Self-compatible Lines of Eruca sativa Mill.by DDRT-PCR
摘要
Abstract
Yunjie is closely related to important vegetables an d oil seed crops,and can be considered a genetic resource for all Brassiceae crops.Self compatibility is an important variant traits to Yunjie.This study aimed to probe the expression organ of self-compatible gene and isolate cDNA fragments related to self-compatibility of Yunjie. Leaf,anther and stigma from self-incompatible (SI)and self-compatible (SC)lines of Yunjie were examined by DDRT-PCR.The results showed that the amplification pattern of leaves and anthers were the same in SI and SC lines.However,different bands were obtained in stigmas of SI and SC lines before and after flowering.SC gene of E. sativa was not showing constitutive expression but showed tissue-specific expression in stigma.Only two fragments were amplified in SC lines before flowering,the one was less than 100 bp,the other was 750 -1 000 bp.A 300 bp fragment was found unique to self-compatible line after flowering.These three cDNA fragments might be closely re-lated to the self-compatibility in Yunjie.In addition,two cDNA fragments were generated in SI line before flowering, one was 500 bp,the other was 750 bp.One band of 500 -600 bp was identified in SI line after flowering.These cDNA fragments could efficiently distinguish self-compatible and self-incompatible lines,and could also be useful for cloning self-compatible gene of Yunjie.关键词
芸芥/自交亲和系/自交不亲和系/DDRT-PCRKey words
Yunjie/Self-compatibility/Self-incompatibility/DDRT-PCR分类
生物科学引用本文复制引用
范惠玲,白生文,李华清,孙万仓..利用 DDRT-PCR 鉴定芸芥自交不亲和系与自交亲和系的差异表达 cDNA[J].华北农学报,2016,31(3):44-50,7.基金项目
甘肃省高等学校科研项目 （）
