华北农学报2016，Vol.31Issue(3)：65-71,7.DOI:10.7668/hbnxb.2016.03.010
梁山慈竹高通量转录组测序及差异表达基因分析
High-throughput RNA-seq and Analysis on Differential Expressed Gene from Dendrocalamus farinosus
摘要
Abstract
The transcriptome from the shoots of Dendrocalamus farinosus and its somatic mutant was sequenced using the RNA-Seq technology to elucidate its functional gene and analyze its differential expressed gene,providing a theoretical basis for its genetic improvement.The sequencing data was assembled by de novo assembly and the dif-ferential expressed gene screened was annotated in COG,GO and KEGG database.In addition,the differential ex-pression of gene related to cellulose and lignin biosynthesis was analyzed,basing on the Swiss-Prot function annota-tion.The sequencing results showed that a total of 86 575 631 reads were produced and assembled into a total of 84 741 unigenes by de novo,among which 49 829 were annotated in Nonredundant protein,Cluster of Orthologous Groups of proteins,Gene Ontology,Kyoto Encyclopedia of Genes and Genomes and Swiss-Prot database.Besides,a total of 3 572 differential expressed unigenes were identified from the plant of seeds (CK)and the somatic mutant No.30.Of these differential expressed genes,757 unigenes had a detailed protein functions in the COG classification system,2 213 unigenes had the function definition in the GO database,385 unigenes were annotated to 94 KEGG Pathways.The expression of genes encoding CesA,Prx,Ubiquitin-conjugating enzyme and Heat shock proteins in-creased in somatic mutant No.30,while,the expression of genes encoding MYB4,4CL,CAD,CCR and LAC de-creased.Our data provide the most comprehensive transcriptomic resource for Dendrocalamus farinosus and the tran-script sequences with important function related to cellulose and lignin biosynthesis were found,which provide the most precious information resources for the further research on bamboo.关键词
梁山慈竹/体细胞突变体/转录组/差异表达基因Key words
Dendrocalamus farinosus/Somatic mutant/Transcriptome/Differential expressed gene分类
生物学引用本文复制引用
王身昌,胡尚连,曹颖,徐刚..梁山慈竹高通量转录组测序及差异表达基因分析[J].华北农学报,2016,31(3):65-71,7.基金项目
国家自然科学基金青年基金项目（31400257；31400333）；四川省“十三五”育种公关资助项目；四川省生物质资源利用与改性工程技术研究中心基金项目（12zxsk07；13zxsk01）；西南科技大学研究生创新基金项目 （）
