华北农学报2016，Vol.31Issue(3)：114-120,7.DOI:10.7668/hbnxb.2016.03.017
蓖麻标雌／单雌／两性系花序 MSAP反应体系的建立
Establish Castor Marked Female Lm Per Female Amphoteric Lineinflores Cence MSAP Reaction System
摘要
Abstract
The purpose of this experiment was to establish Lm-type female system castor different developmen-tal stages marked female,per female,amphoteric system inforescence establish MSAP reaction system.MSAP reac-tion system established to determine differences in inflorescence type genes at different developmental stages of re-search to lay the foundation.The results were as follows:extraction of different types at different developmental sta-ges of inflorescence genomic DNA mixed into the gene pool.EcoRⅠ and HpaⅡ /MspⅠ digested gene pool 12 h. The digestion products and EcoRⅠ,Hpa Ⅱ /MspⅠ adapter overnight connection for pre-amplification.The opti-mized system for the pre-amplification reaction 10 ×PCR Buffer 2.5 μL,dNTPs (10 mmol /L)2.5 μL,Mg2 +(25 mmol /L)2.0 μL,template 2.0 μL,E 0 amplification primer (10 pmol /μL)1 .5 μL,H0 amplification primer (10 pmol /μL)1 .5 μL,LA Taq(5 U /μL)0.25 μL,ddH2 O 12.75 μL.Pre-amplification products were diluted 10-fold for the selective amplification.Selective amplification system optimized for 10 ×PCR Buffer 2.5 μL,dNTPs (10 mmol /L)2.0 μL,Mg2 + (25 mmol /L)4.0 μL,template 3.0 μL,E X amplification primer (10 pmol /μL ) 1 .0 μL,H /Mx amplification primer(10 pmol /μL)1 .0 μL,LA Taq(5 U /μL)0.30 μL,ddH2 O 1 1 .2 μL.关键词
蓖麻/花序/MSAPKey words
Castor/Inflorescence/MSAP分类
农业科学引用本文复制引用
赵永,常旭丰,刘佳琪,朱国立,何智彪,包春光,陈晓凤,姜桐桐,李佳会,黄凤兰,周婷婷..蓖麻标雌／单雌／两性系花序 MSAP反应体系的建立[J].华北农学报,2016,31(3):114-120,7.基金项目
国家自然科学基金项目（30760123；31160290；31460353）；国家农业部公益性行业专项（201003057）；内蒙古自治区高等学校“青年科技英才支持计划”（NJYT-14-A10）；内蒙古自治区科技创新引导项目；内蒙古自治区“草原英才”计划项目；市校合作项目（SXZD2012018；SXZD2012017；SXZD2012006）；内蒙古自治区高校蓖麻产业工程技术研究中心开放基金项目 （）
