华北农学报2016，Vol.31Issue(3)：127-134,8.DOI:10.7668/hbnxb.2016.03.019
促芽肥对头季稻灌浆后期叶片蛋白质表达的影响
Effects of Nitrogen Application for Bud Development on Protein Expression of Flag Leaf at Late Grain Filling Stage in the First Cropping Rice
摘要
Abstract
By using comparative proteomics combined with bioinformatics and related physiological indicators, this paper studied the regulating effects of nitrogen application for bud development on protein expression of flag leaf at late grain filling stage in the first cropping rice with ⅡYou 1273 as a material.Two treatments of urea levels:300.00,0.00 kg /ha applying at 18 d after heading stage were included in the experiment in the first cropping rice. A total of 15 differentially expressed proteins were detected in different fertilizer treatments by the 2D maps of flag leaf proteome.And these proteins were grouped into three expression patterns according to their functions in photo-synthesis carbon assimilation,electron-transfer and photophosphorylation,the defense response to stresses etc..Our results indicated that the proteins involving photosynthesis were down-regulated at late grain filling stage in the first cropping rice.But,nitrogen application for bud development could significantly slow down the decreased amplitude of photosynthesis-related proteins in expression abundance,which relatively enhanced the chlorophyll content and photosynthetic rate of functional leaf and increased the source supply ability at late grain filling stage in the first cropping rice.The result also showed that nitrogen application for bud development could significantly increase the expression abundance of resistance-related protein at late grain filling stage in the first cropping rice,which helped enhance the scavenging of active oxygen,retard the peroxidation of membrane lipid and delay the senescence rate of flag leaf.As a result,the seed-setting rate and yield of the first cropping rice were significantly increased under NA treatment.关键词
再生稻/叶片/促芽肥/蛋白质组学/产量Key words
Ratooning rice/Leaf/Nitrogen application for bud development/Proteomics/Grain-yield分类
农业科技引用本文复制引用
陈鸿飞,张志兴,林文雄..促芽肥对头季稻灌浆后期叶片蛋白质表达的影响[J].华北农学报,2016,31(3):127-134,8.基金项目
国家自然科学基金项目（31401306）；福建省生态学重点学科项目（6112C0611）；福建农林大学科技发展资金项目 （）
