哈尔滨医药2016，Vol.36Issue(3)：248-248,249,2.
干扰素诱导跨膜蛋白抑制小儿流感病毒作用及其机制研究
Interferon Induced Transmembrane Proteins Inhibit Pediatric Influenza Virus Effect and Mechanism
郑健斌 1张剑珲 1陶建平 1司徒勋 1黄莉1
作者信息
- 1. 广州市妇女儿童医疗中心儿科重症监护室，广东 广州510623
- 折叠
摘要
Abstract
Objective To study the interferon induced transmembrane protein to inhibit the action of the pediatric influenza virus and its mechanism. Methods Real time fluorescence quantitative technique carries on the quantitative analysis of gene expression, gene expression of normalized first, and then to adjust the technology of real-time fluorescence quantitative detection conditions, anal-ysis of the stability of the protein gene amplification, sensitivity and specificity, and so on, to build a system method for internal genes, purpose, accurately determine the level of gene expression in the cell, the analysis of the influenza virus and NS protein IF-ITM1/2/3, the relationship between the expression level of and the IFITM1/2/3 of the quantity of protein expression was quantitative detection. Results The A549 cell lines after interferon inducing IFITM1, IFITM2, IFITM3 DNA copy number increased significant-ly. Conclusion IFITM1, IFITM2, IFITM3 NS for influenza virus protein have inhibition effects.关键词
干扰素诱导跨膜蛋白/小儿流感病毒/抑制作用/作用机制Key words
Interferon induced transmembrane protein/Pediatric influenza virus/Inhibition/Mechanism of action分类
医药卫生引用本文复制引用
郑健斌,张剑珲,陶建平,司徒勋,黄莉..干扰素诱导跨膜蛋白抑制小儿流感病毒作用及其机制研究[J].哈尔滨医药,2016,36(3):248-248,249,2.
