郑州大学学报（医学版）2016，Vol.51Issue(4)：458-462,5.DOI:10.13705/j.issn.1671-6825.2016.04.006
妊娠期糖尿病大鼠胰腺组织中 GRP78、TRAF2、Caspase－12蛋白的表达
Detection of expressions of GRP78,TRAF2,and Caspase-12 in pancreatic tissue of gestational diabetes mellitus rats
摘要
Abstract
Aim: To investigate the relationship of endoplasmic reticulum stress ,TRAF2,Caspase-12 and the gestation-al diabetes mellitus(GDM).Methods: A total of 60 healthy female SD rats were randomly allocated into four groups : preg-nant group with high fat and sugar diet (HP),virgin group with high fat and sugar diet(HV),pregnant group with normal di-et(NP),virgin group with normal diet(NV).Fasting blood glucose(FBG),fasting serum insulin(FINS),and blood lipid of each group were tested in the late pregnancy , then the insulin resistance index(HOMA-IR) was calculated.The morpholog-ical changes in pancreatic tissue were observed using HE staining ,the apoptosis of islet cells was detected by TUNEL ,and the expression of GRP78 was tested by immunohistochemistry, the expressions of TRAF2 and Caspase-12 were tested by Western blot.Results: Pregnancy and high fat and sugar diet could increase FBG ,FINS,HOMA-IR,blood lipid synergisti-cally(P <0.05).At the 21st day during pregnancy, pancreatic tissue appeared different degree of atrophy ,islet cells de-creased significantly,pyknosis and karyorrhexis could be seen in HP group .Pregnancy, high fat and high sugar diet were the main factors for increasing the GRP78, TRAF2, Caspase-12 expressions in rat pancreatic tissue , and they had synergis-tic effect(P <0.05).Conclusion: Pregnancy and high fat and sugar diet are involved in ERS ;ERS might be involved in the development of GDM through inducing islet cell apoptosis by TRAF 2-Caspase-12 signaling pathway.关键词
妊娠期糖尿病/内质网应激/凋亡/GRP78/TRAF2/Caspase-12/大鼠Key words
gestational diabetes mellitus/endoplasmic reticulum stress/apoptosis/GRP78/TNF receptor-associated factor 2/Caspase-12/rat分类
医药卫生引用本文复制引用
郎丽翔,辛雅萍,丰树焕,张苏河,付艳芹..妊娠期糖尿病大鼠胰腺组织中 GRP78、TRAF2、Caspase－12蛋白的表达[J].郑州大学学报（医学版）,2016,51(4):458-462,5.基金项目
河南省医学科技攻关计划项目201403091 （）
