华南农业大学学报2016，Vol.37Issue(5)：7-12,6.
猪BMSCs成脂分化中细胞膜钙离子通道、钙敏感受体及成脂定向相关基因表达研究
Expression of plasma membrane calcium channel, calcium-sensing receptor and adipogenic determination genes during the adipogenesis of porcine bone marrow mesenchymal stem cells
摘要
Abstract
[Objective] To investigate the mRNA expression of plasma membrane calcium channel, calci-um-sensing receptor ( CaSR) and adipogenic determination genes during the adipogenesis of porcine bone marrow mesenchymal stem cells ( BMSCs ) . [Method]The porcine BMSCs, isolated and purified from 5-7 d piglet, were induced to adipogenic differentiation. Oil red O staining and triglyceride assay were used to detect the formation of adipocytes. The cells were collected at various time points (0, 1, 2, 5, and 10 d) during the adipogenic differentiation of porcine BMSCs. The mRNA expression patterns of zinc finger protein ( Zfp423 ) , preadipocyte factor 1 ( Pref-1 ) , bone morphogenetic protein 2 ( BMP2 ) , bone morphogenetic protein 4 ( BMP4 ) , plasma membrane calcium channel and CaSR genes were detected by real-time quantitative PCR. [Result]The results of oil red O staining and triglyceride assay indicated that porcine BMSCs were successfully induced to adipogenic differentiation. The findings of real-time quanti-tative PCR demonstrated that the mRNA expression of Zfp423 , Pref-1 , BMP2 and BMP4 significantly in-creased at day 5 ( P<0. 05 ) , suggesting that day 5 is the key time for the adipogenic determination of porcine BMSCs. Meanwhile, the mRNA expression of voltage-dependent alpha-2/delta subunit 1 ( CAC-NA2 D1 ) , calcium release-activated calcium channel modulator 1 ( Orai1 ) , transient receptor potential canonical type 1 ( TRPC1 ) , transient receptor potential melastatin 7 ( TRPM7 ) , transient receptor poten-tial vanilloid receptor 1 ( TRPV1 ) and CaSR also significantly increased at day 5 , implying that calcium channels and CaSR gene might be involved in the adipogenesis of porcine BMSCs. [Conclusion]Our data reveal the expression patterns of plasma membrane calcium channel, CaSR and adipogenic determination genes.关键词
猪骨髓间充质干细胞/钙离子通道/钙敏感受体/成脂定向/Zfp423基因/BMP基因/Pref-1基因Key words
porcine bone marrow mesenchymal stem cells/calcium channel/calcium-sensing receptor/adipogenic determination/Zfp423 gene/BMP gene/Pref-1 gene分类
农业科技引用本文复制引用
叶晶晶,张枫琳,艾玮,朱晓彤,束刚,王丽娜,高萍,江青艳,王松波..猪BMSCs成脂分化中细胞膜钙离子通道、钙敏感受体及成脂定向相关基因表达研究[J].华南农业大学学报,2016,37(5):7-12,6.基金项目
国家自然科学基金(31372397) （31372397）
广东省特支计划项目(2014TQ01N260) （2014TQ01N260）
