华南农业大学学报2016，Vol.37Issue(5)：13-18,6.
G418处理核供体细胞对猪克隆胚胎体外发育效率的影响
Effects of G418 treatment of donor cells on the in vitro developmental efficiency of cloned porcine embryos
摘要
Abstract
Objective] To explore the influence of G418 treatment of donor cells on the developmental ef-ficiency of somatic cell nuclear transfer ( SCNT) embryos.[Method]Porcine adult fibroblasts were collect-ed after six days of G418 treatment. Expression levels of antioxidation and apoptosis-related genes were detected by quantitative RT-PCR before and after G418 treatment. The DNA methylation levels of LINE-1 repetitive sequences and microsatellites were analyzed using bisulfites sequencing analysis, and the in vitro developmental rates of SCNT embryos were investigated.[Result]G418 treatment of donor cells caused sig-nificant (P<0. 05) changes in expression levels of genes related to antioxidation and apoptosis, but did not significantly(P>0. 05) change DNA methylation levels. SCNT embryos cloned from G418-treated do-nor cells exhibited a significantly (P<0. 05) lower in vitro developmental rates compared with the control group.[Conclusion]G418 treatment of donor cell may inhibit the development of SCNT embryo.关键词
G418/猪/克隆/核移植/转基因/胚胎发育Key words
G418/porcine/cloning/nuclear transfer/transgene/embryonic development分类
生物科学引用本文复制引用
李嘉琪,李紫聪,吴珍芳,刘德武..G418处理核供体细胞对猪克隆胚胎体外发育效率的影响[J].华南农业大学学报,2016,37(5):13-18,6.基金项目
863计划项目(2011AA100304) （2011AA100304）
广东省自然科学基金(2014A030313464) （2014A030313464）
