华南农业大学学报2016，Vol.37Issue(5)：24-30,7.
广西普通野生稻遗传多样性中心的确定与核心种质构建
Center of genetic diversity and core collection of common wild rice,Oryza rufipogon Griff.,in Guangxi
摘要
Abstract
[Objective] To confirm genetic diversity distribution center of common wild rice,Oryza rufi-pogon Griff. , in Guangxi, and construct the core collection which will provide a reference for protecting and utilizing wild rice resources in Guangxi. [Method] A total number of 623 common wild rice re-sources from Yujiang River Basin,Hongshui River Basin,Nanliujiang River Basin and Guibei Mountain were used to study the genetic diversity with 24 SSR markers. The stepwise cluster analysis was used to construct 10% and 5% core collections of common wild rice in Guangxi. [Result]There were 114 alleles to be detected at 24 SSR loci. The number of alleles per locus (A) was 4. 75, the average number of ef-fective alleles (Ae) was 3. 000 1, the Shannon-weaver information index (I) was 1. 180 1 and the aver-age expected heterozygosity ( He ) was 0. 638 8. The order of genetic diversity of 9 regional populations was Yongning ( YN) > Lingui ( LG) > Fusui ( FS) > Rongxian ( RX) > Guigang ( GG) > Pingnan ( PN) > Gupeng ( GP) > Wulitang ( WLT) > Bobai ( BB) . The order of genetic diversity of four re-gions was Yujiang River Basin>Guibei Mountain > Nanliujiang River Basin > Hongshui River Basin. There were 31 copies belong to 5% core collection,including 14 copies of Yongning population and 12 copies of Fusui population which accounted for 5. 76% and 18. 75% of analyzed samples respectively.[Conclusion]The genetic diversity center of Guangxi common wild rice is Yujiang River Basin. Wild rice of Yongning is widely distributed and presents various types. Wild rice of Fusui shows great rich genetic diversity. Yongning and Fusui regional populations,which are main sources of the core collection sam-ples,are considered to be the most important populations of Guangxi common wild rice, and should be paid particular attention to their protection and exploitation.关键词
普通野生稻/SSR/遗传多样性/核心种质/广西Key words
Oryza rufipogon/SSR/genetic diversity/core collection/Guangxi分类
农业科技引用本文复制引用
薛艳霞,梁燕理,冯璇,黄金艳,刘芳,覃宝祥,邱永福,李容柏..广西普通野生稻遗传多样性中心的确定与核心种质构建[J].华南农业大学学报,2016,37(5):24-30,7.基金项目
广西科学技术与开发项目(桂科重14121001-1-5) （桂科重14121001-1-5）
广西研究生教育创新计划项目(T3310098601) （T3310098601）
广西大学科研基金(XDZ110082) （XDZ110082）
