华南农业大学学报2016，Vol.37Issue(5)：31-37,7.
转cry1 Ab13基因玉米新种质的创制
Creation of new transgenic maize germplasm harboring cry1 Ab13 gene
摘要
Abstract
Objective] To construct a plant expression vector including a lepidopteran-resistant gene cry1 Ab13 and use it to create a new transgenic maize germplasm with high resistance to Ostrinia furnaca-lis.[Method]The cry1Ab13 gene was inserted into expression plasmid pCAMBIA3300-Bar by homologous recombination method,and the plant expression vector pCAMBIA3300-cry1Ab13-Bar with Bar gene as a selection marker was obtained. Following Agrobacterium mediated transformation of the immature embryo of maize inbred line H99 , the regenerated plants were detected by PCR and herbicide-resistance selection for each generation. T2 generation plants were also detected by Southern blotting and real-time quantita-tive PCR. The resistance of transgenic plants to O. furnacalis was tested indoor and in the fields.[Result]A plant expression vector containing the cry1 Ab13 gene was successfully constructed and three T2 transgenic maize with high resistance and one T2 transgenic maize with resistance to O. furnacalis were obtained. The cry1Ab13 gene was integrated into maize genome as confirmed by Southern blotting, and it was expressed in maize as shown by real-time quantitative PCR. The resistance of transgenic plants to O. furnacalis was improved significantly compared to non-transgenic control.[Conclusion]We introduced the cry1Ab13 gene into maize and its expression significantly improved the resistance of maize to O. furnaca-lis, which will promote the development of new insect-resistant maize germplasm.关键词
抗虫性/cry1Ab13基因/转基因玉米/玉米螟Key words
insect resistance/cry1 Ab13 gene/transgenic maize/Ostrinia furnacalis分类
农业科技引用本文复制引用
陈沼汀,董昭旭,刘双,孙国旭,李晖,魏晓禹,孙苏,关淑艳..转cry1 Ab13基因玉米新种质的创制[J].华南农业大学学报,2016,37(5):31-37,7.基金项目
吉林省省级粮食生产发展专项(2015001) （2015001）
吉林农业大学科研启动基金(201242) （201242）
