华南农业大学学报2016，Vol.37Issue(5)：91-97,7.
16年生大叶相思高效芽诱导、增殖体系研究
Efficient bud inducing and proliferation of 16-year-old Acacia auriculiformis plant
摘要
Abstract
Objective] To utilize the superior properties of mature Acacia auriculiformis, improve propa-gation efficiency, and accelerate breeding and promotion of elite varieties.[Method] A. auriculiformis bud inducing system was established by comparing the disinfection strategies for the explants from 16-year-old A. auriculiformis and screening the bud inducing media. The proliferation media were screened and the relationship between subculture times and proliferation rate was studied. [Result] The optimal explants were those collected from the 3th-5th node of shoots in August. For the optimal explants treated with 0. 1% mercuric chloride for 18 min and 75% ethanol for 15 s, and inoculated onto the best bud in-ducing medium containing MS medium + 6-BA 1. 0 mg·L-1 +sucrose 40 g·L-1 , the germination rate reached 92. 00%. When type II medium ( MS+ 6-BA 1. 0 mg·L-1 + NAA 0. 1 mg·L-1 +Ac 0. 05 g·L-1 +sucrose 30 g·L-1 ) was used for proliferation, the proliferation rate was high and stable with an average rate of 2. 63 for seven generations. [Conclusion] This tissue culture system enables fast prop-agation for mature elite plants of A. auriculiformis.关键词
大叶相思/成年优树/组织培养/芽诱导Key words
Acacia auriculiformis/mature elite plant/tissue culture/bud inducing分类
林学引用本文复制引用
王鸿,黄烈健,胡峰..16年生大叶相思高效芽诱导、增殖体系研究[J].华南农业大学学报,2016,37(5):91-97,7.基金项目
“十二五”国家科技支撑计划项目(2012BAD01B0402)。 （2012BAD01B0402）
