湖南中医药大学学报2016，Vol.36Issue(7)：5-10,6.DOI:10.3969/j.issn.1674-070X.2016.07.002
基于iTRAQ技术的冠心病血瘀证蛋白质谱研究
Study of Protein Mass Spectrometry of Coronary Heart Disease with Blood Stasis Syndrome Based on iTRAQ Technology
摘要
Abstract
Objective To explore the differential protien mass spectrum of coronary heart disease (CHD) with blood stasis syndrome, and its biological mechanism. Methods The diffrential proteins in blood stasis syndrome and non-blood stasis syndrome groups were isolated and identified by isobaric tags for relative and absolute quantitation (iTRAQ) technology. Results 780 proteins were identified. In the two groups, the expression difference of 27 protein spots was 1.5 times higher, and 11 of them were up-regulated and 16 of them were down-regulated. The up-regulated diffrential proteins were related with immune response, blood coagulation and lipid metabolism. The function of down-regulated proteins was related to platelet cytomorphosis, metastasis between cells, Conclusion The CHD with blood stasis syndrome has close relation with immune response, platelet participate in the coagulation reaction of hyperthyroidism, as well as with the influence of platelet morphology change, platelets, vascular endothelial cells and platelets adhesion and migration.关键词
冠心病血瘀证/iTRAQ/蛋白质质谱/差异蛋白Key words
coronary heart disease with blood stasis syndrome/iTRAQ/protein mass spectrometry/differential proteins分类
医药卫生引用本文复制引用
肖隋熙,李杰,袁肇凯,李琳,王建国,胡志希,孙贵香,袁秀芳,简维雄..基于iTRAQ技术的冠心病血瘀证蛋白质谱研究[J].湖南中医药大学学报,2016,36(7):5-10,6.基金项目
国家自然科学基金项目(81202647),中国博士后基金资助（2013M530355）。 （81202647）
