湖南中医药大学学报2016，Vol.36Issue(7)：11-19,9.DOI:10.3969/j.issn.1674-070X.2016.07.003
辣椒VQ基因家族的鉴定与低温胁迫下的表达分析
Identification and Low Temperature-induced Expression Analysis of VQ Gene Family in Pepper
摘要
Abstract
Objective To identify VQ genes from pepper genome, and analyze the gene structure, phylogeny, chromosome location, tissue expression pattern and low temperature-induced expression of CaVQ genes. Methods The CaVQ genes were identified by pepper genome database and bioinformatic method. Gene structure and chromosome localization were analyzed by softwares, such as MEME, GSDS 2.0, DNAMAN 8, MapInspect etc. A phylogenetic tree was created by using the MEGA 6.0 program. Tissue expression pattern and expression under low temperature stress of CaVQ genes were analyzed based on RNA-seq database and qRT-PCR method. Results 29 CaVQ genes were identified from pepper. The structure of CaVQ genes were relatively simple, and distributed on different chromosomes unevenly. CaVQ genes were divided into 2 groups (Group I and Group II) and 6 subgroups (Group I a, Group I b, Group I c, Group II a, Group II b and Group IIc). The expression of this gene family had tissue specificity, and the low temperature stress could change the expression of partial CaVQ genes. Conclusion CaVQ gene family consisted of 29 genes. They were devided into 2 groups, 6 subgroups and distributed on 10 chromosomes with special response to external stimulates and different expression patterns in different tissues and developmental stages.关键词
辣椒/VQ基因家族/鉴定/低温胁迫表达分析Key words
pepper/VQ gene family/identification/low temperature-induced expression analysis分类
医药卫生引用本文复制引用
张亚利,谢玲玲,欧阳娴,樊悦,罗娟,童巧珍,刘峰..辣椒VQ基因家族的鉴定与低温胁迫下的表达分析[J].湖南中医药大学学报,2016,36(7):11-19,9.基金项目
国家自然科学基金项目(31470105)；湖南省自然科学基金项目（2015JJ2089）。 （31470105）
