湖南中医药大学学报2016，Vol.36Issue(7)：20-24,5.DOI:10.3969/j.issn.1674-070X.2016.07.004
加味丹参饮预处理调控自噬相关基因Beclin-1和Atg5表达抗大鼠心肌缺血再灌注损伤
Pretreatment with Modified Danshen Decoction against Myocardial Ischemia Reperfusion Injury in Rats by Regulating Autophagy Related Genes Beclin-1 and Atg5
摘要
Abstract
〔Abstract〕 Objective To investigate the pretreatment with Jiawei Danshen decoction against myocardial ischemia reperfusion injury in rats whether by regulating Beclin-1 and Atg5 expression of autophagy. Methods The 60 healthy SD rats were randomly divided into control (C) group, sham-operation (S) group, ischemia reperfusion injury (IRI) group, IRI+Jiawei Danshen decoction (JDS) group, IRI+JDY+Inhibitor (I) group, 12 rats in each group. The IRI model was established by ligating-relaxing the left anterior descending coronary of rats. Myocardial infarction rate was measured by using triphenyltetrazolium chloride (TTC) staining. The autophagic vacuoles were observed via transmission electron microscope (TEM). The mRNA expression of autophagy related gene Beclin-1 and Atg5 were detected by real-time quantitative reverse transcription- polymerase chain reaction (RT-PCR), and the expression changes of myocardial Beclin-1 protein were detected by Western blot. Results The infarction rate inIRI+JDY group was significantly smaller than that in IRI group and IRI+JDY+I group (P<0.01). TEM showed that in IRI+JDY group, autophagy of cardiomyocytes could be regulated moderately and the structure of cardiomyocytes could be improved. The expression levels of Beclin-1 and Atg5 mRNA, and Beclin-1 protein in myocardium of rat in IRI group were significantly higher than SG group (P<0.01). The expression levels of Beclin-1 and Atg5 mRNA, and the expression of Beclin-1 protein in myocardium of rats in IRI+JDY group and IRI+JDY+I group were decreased significantly compared with the IRI group (P<0.01). The expression levels of Beclin-1 and Atg5 mRNA, and Beclin-1 protein in myocardium of rats with IRI+JDY group were significantly higher than that in IRI+JDY+I group (P<0.01). Conclusion The modified Danshen decoction can regulate the autophagy of ischemia reperfusion cardiomyocytes and protect the cells by regulating the autophagy related genes (Beclin-1 and Atg5).关键词
加味丹参饮/心肌缺血再灌注/自噬/Beclin-1/Atg5/丹参/檀香/川芎Key words
modified Danshen decoction/myocardial ischemia reperfusion/autophagy/Beclin-1/Atg5/Salvia miltiorrhiza/sandalwood/Chuanxiong分类
医药卫生引用本文复制引用
彭霞,黄政德,成细华,任婷,孙彦波,饶春梅..加味丹参饮预处理调控自噬相关基因Beclin-1和Atg5表达抗大鼠心肌缺血再灌注损伤[J].湖南中医药大学学报,2016,36(7):20-24,5.基金项目
国家自然科学基金资助项目(81373576，81503536)；湖南省教育厅项目（14B136，14C0872）；湖南省中医药管理局重点课题(201304)；湖南省科技厅一般项目（2014SK3034）；湖南省教育厅优秀青年项目（14B136）；中西医结合防治心脑疾病的相关基础研究湖南省高校科技创新团队科研项目。 （81373576，81503536）
