湖南中医药大学学报2016，Vol.36Issue(7)：25-27,3.DOI:10.3969/j.issn.1674-070X.2016.07.005
养心通脉方对大鼠心肌缺血损伤模型心肌细胞线粒体功能的影响
Effect of Yangxin Tongmai Formula on Mitochondrial Function of Cardiomyocyte in Myocardial Ischemia Injury Rat Models
摘要
Abstract
Objective To evaluate the effect of Yangxin Tongmai Fang on mitochondrial respiration of cardiomyocyte in the isoproterenol-induced myocardial ischemia injury rats model. Methods The experimental rats were divided into three groups:control group, model group, Yangxin Tongmai Fang group, 15 rats in each group. After the experimental period, the animals were sacrificed by cervical decapitation. The mitochondrial respiration of cardiomyocyte was evaluated by the levels of respiratory control ratio (RCR), ADP/O ratio and ATP concentration. The mitochondrial cholesterol, phospholipids (PL), triacylglycerol(TG) and free fatty acids (FFAs) were also determined. Results The levels of RCR, ADP/O ratio and ATP concentration in model group were lower than that in control group (P<0.001), the RCR, ADP/O ratio and ATP concentration in Yangxin Tongmai group were close to the control group (P>0.05). Compared with the control group, the levels of cholesterol, TG and FFAs in model group were significantly increased (P<0.001), and the PL content was decreased obviously (P<0.001). But there were no significant difference in the levels of cholesterol, TG, FFAs and PL between Yangxin Tongmai Fang group and control Group (P>0.05). Conclusion Yangxin Tongmai Fang shows protective effect on mitochondrial respiration and its membrane integrity of cardiomyocyte in the isoproterenol-induced myocardial injury rat models.关键词
养心通脉方/心肌缺血/心肌细胞/线粒体功能/呼吸控制率/二磷酸腺苷磷/氧比值Key words
Yangxin Tongmai Fang/myocardial ischemia/cardiomyocyte/mitochondrial function/respiratory control rate/ADP/O ratio分类
医药卫生引用本文复制引用
黄正新,黄献平,袁肇凯..养心通脉方对大鼠心肌缺血损伤模型心肌细胞线粒体功能的影响[J].湖南中医药大学学报,2016,36(7):25-27,3.基金项目
国家自然科学基金项目（81273670）；湖南省教育科学“十二五”规划课题资助（XJK011BGD011）；中医诊断学国家重点学科开放项目（2013ZYZD16）。 （）
