湖南中医药大学学报2016，Vol.36Issue(8)：17-21,5.DOI:10.3969/j.issn.1674-070X.2016.08.005
健脾补土法组方含药血清对体外嗅鞘细胞低氧/复氧损伤模型P65、P50及磷酸化IκBα表达的影响
Effect of Spleen-Strengthening Therapy Contained Serum on P65, P50 and P-IκBα Expression in Olfactory Ensheathing Cells with Hypoxia/Reoxygenation Injury Models
摘要
Abstract
Objective To investigate the effect of spleen-strengthening therapy contained serum on subgene P65, P50 of nudear factor-kappa B (NT-KB) and P-IκBα expression in olfactory ensheathing cells with hypoxia/reoxygenation injury. Methods Primary cultured olfactory ensheathing cells were randomly divided into five groups: normal serum control group, normal serum+hypoxia group, hypoxia+edaravone contained serum group, hypoxia+spleen-strengthening therapy contained serum group, normal serum+hypoxia+NF-KB inhibitor/antioxidant (PDTC 50 μmol/L) group. The cerebral ischemia-reperfusion injury model of olfactory ensheathing cells were induced by hypoxia/reoxygenation of micro-aerobic bags method. Then, the cytoplasm P65, P50, P-IκBα and nuclear P65, P50 content in olfactory ensheathing cells were detected by Western blot assay. Results Compared with normal serum control group, the cytoplasm and nucleus P65, P50, cytoplasm P-IκBαexpression in normal serum+hypoxia group were significantly increased, the difference was statistically significant (P<0.01). Compared with normal serum control group, the cytoplasm and nucleus P65, P50, cytoplasm P-IκBα expression were lower in hypoxia+edaravone group, hypoxia+Jianpi Butu group, normal serum+hypoxia+PDTC group, hypoxia+Jianpi Butu+PDTC group, the difference was statistically significant (P<0.05). Conclusion Spleen-strengthening therapy can prevent the activation of NF-κB signal path, which may be due to its inhihitory effect on P65/P50 and P-IκBα overexpression in olfactory ensheathing cells with hypoxia/reoxygenation injury.关键词
健脾补土法/嗅鞘细胞/低氧/复氧损伤模型/P65/P50/P-IκBαKey words
spleen-strengthening therapy/olfactory ensheathing cells/hypoxia/reoxygenation injury model/P65/P50/P-IκBα分类
医药卫生引用本文复制引用
李路迢,刘旺华,李花,彭智远,周翠玲,付小金..健脾补土法组方含药血清对体外嗅鞘细胞低氧/复氧损伤模型P65、P50及磷酸化IκBα表达的影响[J].湖南中医药大学学报,2016,36(8):17-21,5.基金项目
国家自然科学基金（81202632、81473567），教育部博士点基金（20124323120003），湖南省自然科学基金（13JJ3097），湖南省教育厅科研项目（14B134、15K092）。 （）
