湖南中医药大学学报2016，Vol.36Issue(8)：22-25,4.DOI:10.3969/j.issn.1674-070X.2016.08.006
补肾祛痰活血汤对缺血再灌注损伤大鼠Occludin、 ZO-1蛋白的调节作用
The Effect of Bushen Huoxue Decoction through Regulating ZO-1 and Occludin Proteins in Ischemia Reperfusion Injury Model Rats
摘要
Abstract
〔Abstract〕 Objective To investigate the effect of Bushen Huoxue decoction on rat brain edema, blood brain barrier permeability, damage zone occlusal protein (occludin), zonula occludens (ZO-1) protein in pretreatment ischemia reperfusion injury rats. Methods Fifty SD adult male rats were divided into normal group, model group, sham operation group, treatment group, control group according to the random number table method, 10 rats in each group. The treatment group was given Bushen Huoxue decoction, while the control group was given Xiaoshuan Tongluo capsule. The normal group, model group, sham operation group were given the corresponding double distilled water. The model of cerebral ischemia-reperfusion injury was established by suture method. After 72 hours, the brain water content was determined by dry wet weight method to reflect the degree of brain edema. The damage zone occludin and ZO-1 protein expression were tested by immunohistochemical method, and the results were analyzed by correlation analysis. Results Compared with the normal group and sham operation group, the brain tissue water content in rats at 72 h of model group, treatment group and control group was increased, the occludin and ZO-1 protein expression in brain tissue damage zone were significantly decreased (P<0.01). The water content of brain tissue was positively correlated with the degree of brain tissue injury, ZO-1 and occludin protein were negatively correlated with the degree of brain tissue injury. Conclusion Bushen Quyu Huoxue decoction can inhibite the decrease of occludin and ZO-1 protein, inhibite the blood brain barrier permeability and relieve brain edema formation.关键词
补肾祛痰活血汤/咬合蛋白/闭锁小带蛋白/脑缺血再灌注损伤模型/制附子/桂枝/熟地黄Key words
Bushen Huoxue decoction/occludin/zonula occludens protein/cerebral ischemia reperfusion injury model/prepared monkshood/Cassia Twig/prepared rehmannia root分类
医药卫生引用本文复制引用
杨涛,王净净,陶琳,钟乔青,吴彬才..补肾祛痰活血汤对缺血再灌注损伤大鼠Occludin、 ZO-1蛋白的调节作用[J].湖南中医药大学学报,2016,36(8):22-25,4.基金项目
衡阳市科学技术局科技计划发展项目项目编号（2014KJ63）。 （）
