湖南中医药大学学报2016，Vol.36Issue(9)：8-12,5.DOI:10.3969/j.issn.1674-070X.2016.09.002
左归降糖益肾方含药血浆对高糖培养小鼠足细胞凋亡的影响
Effects of Zuogui Jiangtang Yishen Decoction Plasma on Apoptosis of Podocyte Cells Cultured in High Glucose Condition
摘要
Abstract
〔Abstract〕 Objective To investigate the expression levels of bax, bcl-2 and cell apoptosis in podocyte cells cultured in high glucose condition in vitro, and the regulation effects of Zuogui Jiangtang Yishen decoction plasma. Methods Cultured podocyte cells of mouses were divided into four groups: blank group (group A, 25 mmoL/L glucose, 10% blank plasma), high glucose group (group B, 200 mmoL/L glucose, 10% blank plasma), Zuogui Jiangtang Yishen decoction plasma group (Group C, 200 mmoL/L glucose, 10% containing medicine plasma), and 4-Phenylbutyric acid (4-PBA) group (group D, 200 mmoL/L glucose, 10% containing medicine plasma). The cell apoptosis were observed by immunofluorescence stainings. The cell viability was detected by MTT colorimetry assay. The protein and mRNA expressions of bax and bcl-2 were analyzed by Western blot or reverse transcription-polymerase chain reaction, respectively. Results Compared withthe group A, the viability of podocyte cells was significantly decreased of group B, and their expressions of bax and bcl-2 protein or mRNA were upregulated (P<0.01). Compared with group B, the expressions of bax protein or mRNA of podocyte cells were downregulated (P<0.05 or P<0.01), but the protein or mRNA of bcl-2 were upregulated (P<0.01). Compared with group C, the cell viability of group D was significantly upcreased (P<0.05), but there was no statistical significance of their expressions of bax and bcl-2 protein or mRNA (P>0.05). Conclusion The higher level expressions of bax and bcl-2 protein or mRNA were the molecular mechanisms of podocyte injury. ZJYD plasma can repair the damaged podocyte by regulateing the protein or mRNA expressions of bax and bcl-2.关键词
左归降糖益肾方/高糖/足细胞/bax/bcl-2Key words
Zuogui Jiangtang Yishen decoction/high glucose/podocyte/bax/bcl-2分类
医药卫生引用本文复制引用
陈聪,罗文娟,喻嵘,刘慧萍,杨胜辉,唐元,曾婧,张翔..左归降糖益肾方含药血浆对高糖培养小鼠足细胞凋亡的影响[J].湖南中医药大学学报,2016,36(9):8-12,5.基金项目
国家自然科学基金面上项目（81273753）；湖南省自然科学基金（12JJ2048，12JJ9031）；湖南省高校创新平台开放基金（14K069）；细胞生物学与分子技术湖南高校重点实验室；“心脑疾病中西医结合防治研究”湖南省高校科技创新团队资助；贵阳中医学院2015年院内博士启动基金资助。 （）
