湖南中医药大学学报2016，Vol.36Issue(9)：21-25,5.DOI:10.3969/j.issn.1674-070X.2016.09.005
灵仙通络方对C518大鼠膝关节退变软骨细胞增殖、Ⅱ型胶原及基质金属蛋白酶-13表达的影响
Effect of Lingxian Tongluo Prescription on the Expression of Cartilage Cell, COL-II and MMP-13 in C518 Rats with Knee Joint Degeneration
摘要
Abstract
〔Abstract〕 Objective To investigate the effect of Lingxian Tongluo prescription on the expression of cartilage cell, COL-II and MMP-13 in C518 rats with knee joint degeneration. Methods Selected C518 rat knee cartilage cell line as the object, which were randomly divided into the Chinese medicine group (Lingxian Tongluo decoction) and Western medicine group (indometacin and glucosamine) and control group (sodium chloride solution), and then the above groups were treated with culture and induced degeneration. On the 1st d, 2nd d, 3rd d after administration, and then the expression of cartilage cell, COL-II and matrixmetalloproteinase-13 (MMP-13) in rats with knee joint degeneration was detected. Results At the same time point, the MTT values of the Traditional Chinese medicine group and Western medicine group had significant difference (P<0.01). The MTT values of the control group were not statistically significant (P>0.05). Compared with the Western medicine group and the control group, the COL-II immunohistochemical staining of Traditional Chinese medicine group were statistically significant (P<0.01), and the Traditional Chinese medicine group was better than the Western medicine group (dyeing index χ2=16.26, df=2, P<0.01). The MMP-13 immunohistochemistry staining in the control group was significant comparing with the Traditional Chinese medicine group and the Western medicine group, with statistical significance (P<0.05), and the Western medicine group compared with the Traditional Chinese medicine group was significantly different (P<0.05), and the traditional Chinese medicine group was better than that of the Western medicine group (dyeing index χ2=54.31, df=2, P<0.05). Conclusion The knee degenerative cartilage cells of C518 rats intervened by Lingxian Tongluo prescription can promote its proliferation, regulate the expression of COL-II and MMP-13, and delay the development of knee osteoarthritis disease.关键词
膝关节退变/软骨细胞/Ⅱ型胶原/基质金属蛋白酶-13/软骨细胞增殖/灵仙通络方Key words
knee joint degeneration/cartilage cells/collagen Type Ⅱ/matrixmetalloproteinase-13/cartilage cell prolifera-tion/Xianling Tongluo prescription分类
医药卫生引用本文复制引用
林强,祁文兵,白明华,王明怀,于继岗,彭页..灵仙通络方对C518大鼠膝关节退变软骨细胞增殖、Ⅱ型胶原及基质金属蛋白酶-13表达的影响[J].湖南中医药大学学报,2016,36(9):21-25,5.基金项目
中国博士后科学基金第八批特别资助（2015T81110）。 （）
