杨春萍 1刘丽芳 2杨涛 1聂佳欣 1高双双 1胡文文3
作者信息
- 1. 湖南中医药大学，湖南 长沙 410208
- 2. 湖南中医药大学第一附属医院，湖南 长沙 410007
- 3. 湖南省第二人民医院，湖南 长沙 410007
- 折叠
摘要
Abstract
〔Abstract〕 Objective To observe the effect of Chaishao Rupi Granules on B type ultrasound images and histomorphology of rabbit models with breast hyperplasia. Methods 10 rabbits were randomly selected from 56 healthy unfertilized female New Zealand rabbits as the normal control group, which were given muscle injection with normal saline, 46 rabbits as model preparation group, which were taken female progestogen sequential muscle injection. Randomly killing female rats in the two groups, determine the model is successful or not by observing the pathological section. The remaining 45 rabbits in model preparation group were divided into 5 groups:model control group, Rupi Sanjie granule group, and low dose, middle dose, high dose of Chaishao Rupi granules groups. The six groups were administrated normal saline, 0.56 g/kg Rupi Sanjie granules, and 0.47 g/kg, 0.94 g/kg, 1.88 g/kg Chaishao Rupi granules, respectively, once a day, continuous for 3 months. At 24 h after the last lavage, we implemented the anesthesia to all of the rabbits and collected the second pair of breasts' B ultrasound images. We cut the second pair of breast tissue out. The histomorphology after HE staining was observed. The numbers of lobular acini, duct epithelial layer and acinus cavity diameter were counted. Results Chaishao Rupi granules can shrink the rabbit model's breast tissue keyed acoustic area, decrease internal echo intensity, the effect of high dose group is the most obvious. Compared with model group, three doses of Chaihu Rupi granules and Rupi Sanjie granules can narrow the acinus cavity diameter significantly (P<0.01). The middle dose and high dose can reduce the mammary ductal epithelial cell layers(P<0.05), low dose can also reduce lobular acini number (P<0.05). Conclusion Chaishao Rupi granules can improve B ultrasonicimage of mammary gland hyperplasia rabbit models, can reduce the degree of female rabbits' hyperplasia of mammary glands. The effect on hyperplasia of mammary glands is obvious.关键词
乳腺增生/柴芍乳癖颗粒/B超声像/组织形态学/柴胡Key words
hyperplasia of mammary glands/Chaishao Rupi granules/B type ultrasound images/histomorphology分类
医药卫生
