湖南中医药大学学报2016，Vol.36Issue(9)：36-39,4.DOI:10.3969/j.issn.1674-070X.2016.09.009
补阳还五汤及其四类有效部位对脊髓损伤大鼠大脑运动皮质的神经保护作用
The Neuroprotective Effect of Buyang Huanwu Decoction and its Four Kinds of Active Fractions on Cerebral Motor Cortex in Spinal Cord Injury Rats
摘要
Abstract
〔Abstract〕 Objective To observe the effect of Buyang Huanwu decoction (BYHWD) and its four kinds of active fractions (alkaloid, glycoside, polysaccharide and aglycone) on cerebral motor cortex in spinal cord injury (SCI) rats, and then explore the proper mechanism of the neuroprotection. Methods A total of 48 adult Sprague-Dawley rats were randomly divided into eight groups: control group, sham-operation group, SCI group, BYHWD group, alkaloid group, glycoside group, polysaccharide group and aglycone group (n=6). SCI model was performed by cutting the right half spinal cord at the level of T3-T4. Motor function of lower limb was assessed with Basso-Beattie-Bresnahan (BBB) scale at the following moments respectively: 1 d before operation, and 1 d, 1 w, 4 w and 8 w after operation. Ratio of cell apoptosis in cerebral motor cortex was detected by TUNEL staining after 8w treatment. Results Compared with the control group and sham-operation group, the BBB scores in SCI and all medicine intervention groups decreased significantly (P<0.01). After four weeks of operation, the BBB scores in BYHWD and alkaloid groups were significantly higher than the SCI group (P<0.05). After eight weeks of operation, the BBB scores in BYHWD, alkaloids and glycoside groups were higher than that in SCI group (P<0.05). After eight weeks of operation, the ratio of cell apoptosis in SCI and all medicine intervention groups was significantly higher than the normal and sham-operation groups (P<0.01). The ratio of cell apoptosis in BYHWD, alkaloid and glycoside groups was lower than that in SCI group (P<0.01 or P<0.05); while ratio of cell apoptosis in alkaloid, glycoside, polysaccharide andaglycone groups were significantly higher than that in BYHWD group (P<0.01 or <0.05). Conclusion BYHWD can promote the recovery of the motor function in SCI rats. The alkaloids and glycoside may be the most important important material base of BYHWD. Its mechanism may be associated with inhibiting neuronic apoptosis of SCI rats.关键词
脊髓损伤/补阳还五汤/运动功能/细胞凋亡Key words
spinal cord injury/Buyang Huanwu decoction/motor function/cell apoptosis分类
医药卫生引用本文复制引用
黄胜,袁莉,陈安,殷坚,潘波,谷陟欣,李亮..补阳还五汤及其四类有效部位对脊髓损伤大鼠大脑运动皮质的神经保护作用[J].湖南中医药大学学报,2016,36(9):36-39,4.基金项目
国家自然科学基金资助项目（81302899）；湖南省科技厅科技计划重点项目（2013SK2023）；湖南省中医药科研计划项目（2013111）；湖南中医药大学中医诊断学国家重点学科开放基金（2014-37）。 （）
