湖南农业大学学报（自然科学版）2016，Vol.42Issue(4)：409-414,6.
葡萄牙牡蛎外套膜转录组测序及壳色基因挖掘
Transcriptome analysis and shell gene mining on Crassostrea angulata
摘要
Abstract
Shell coloration is one of important traits for genetic breeding, for improving genome structure and explore new potential gene and further determine the genetic structure of golden shell gene of oyster, the transcriptome sequencing of Crassostrea angulata with black shell and golden shell mantle were performed by Illumina platform. Data were obtained from black shell and golden shell transcriptome were 5 857 718 092 nt and 6 727 447 326 nt respectively, out of which 58 004 560 and 66 614 854 clean reads were remained after screening, respectively. To compare with the database from COG, GO, KEGG, Swiss-Prot and NR, new gene numbers corresponding to each database were 147, 397, 203, 567, and 1 347 respectively. To further explore the shell color genes of oyster, functional annotation, clustering analysis and screening from the reported shell matrix proteins on the differential expression gene (DEGs), 14 genes related to shell color expression were identified out and 5 down-regulated genes out of them might be related to the expression of golden shell.关键词
葡萄牙牡蛎/壳色/转录组Key words
Crassostrea angulata/shell pigmentation/transcriptome分类
生物科学引用本文复制引用
严璐琪,郭香,巫旗生,祁剑飞,宁岳,王晓清,曾志南..葡萄牙牡蛎外套膜转录组测序及壳色基因挖掘[J].湖南农业大学学报（自然科学版）,2016,42(4):409-414,6.基金项目
现代农业产业技术体系建设专项(CARS-48)；福建省种业工程项目(201451477-9)；福建省属公益类科研院所基本科研专项(2015R1003-8) （CARS-48）
