湖南农业大学学报（自然科学版）2016，Vol.42Issue(4)：419-423,5.
1株小鼠肠道酵母菌的分离与鉴定
Isolation and identification of a yeast strain from intestine of mice
摘要
Abstract
In order to develop a new type of probiotics, a yeast strain was isolated from intestine of mice, and it was identified using the methods of morphology, physiology, biochemistry, gene sequencing and phylogenetic tree. The results showed that the bacterial colony presented in white milky color and oval shape. Through microscopic observation, we found its cell presented in oval shape, and the asexual reproduction was gemmation. The yeast could ferment glucose, sucrose and D–mannitol, as well as assimilate peptone and potassium nitrate, while, it could not tolerate high concentration of alcohol, and the urease test was negative. The results from 18S rDNA sequencing analysis showed that DNA homology of the strain (Accession No. KC534843) and Debaryomyces hansenii (Accession No. AB013567) reached up to 99%, moreover, 26S rDNA sequencing analysis showed that the homology between the strain (Accession No. KC534842) and Debaryomyces hansenii (Accession No. EU131182) was also 99%. From the result of phylogenetic tree showed that 18s rDNA gene sequence of the strain was closest to Debaryomyces hansenii (Accession No. AB013590), and 26S rDNA gene sequence of the strain was closest to Debaryomyces hansenii (Accession No. EU131182). We could draw the conclusion that the yeast strain was Debaryomyces hansenii.关键词
小鼠/肠道酵母菌/汉逊德巴利酵母/26S rDNA/18S rDNAKey words
mice/intestinal yeast/Debaryomyces hansenii/26S rDNA/18S rDNA分类
生物科学引用本文复制引用
肖新云,刘又嘉,邓艳玲,郭抗萧,谭周进..1株小鼠肠道酵母菌的分离与鉴定[J].湖南农业大学学报（自然科学版）,2016,42(4):419-423,5.基金项目
国家自然科学基金项目(81573951)；长沙市科技局项目(K1508025-21) （81573951）
