湖南农业大学学报(自然科学版)2016,Vol.42Issue(4):429-434,6.
3个群体方斑东风螺线粒体 COI 基因的遗传多样性分析
Genetic polymorphisms of three different groups of Babylonia areolata based on COI gene
摘要
Abstract
We analyzed and compared the polymorphism of mitochondrial COI gene sequence derived from three different groups of Babylonia areolata in Dongshan, Zhanjiang and Beihai by means of PCR. The results showed that the nucleotide sequence of COI gene was 680 bp with a high percentage of A+T base composition (about 62.42%); there were 32 singleton poylmorphic sites, 0.707±0.092 haplotype diversity, 0.001 85±0.000 39 nucleotide polymorphisms index, and 1.252 average number of nucleotide differences. Taking Babylonia lutosa and Nassarius as outgroups, we built NJ phylogenetic tree, its topology suggested that all the Babylonia areolata clustered together first, then mixed with Babylonia lutosa for one, and finally Nassarius formed independent.关键词
方斑东风螺/COI 基因/单倍型/遗传多样性Key words
Babylonia areolata/COI gene/phyletic evolution/genetic polymorphism分类
生物科学引用本文复制引用
朱丹丽,王晓清,曾志南,秦溱,熊钢,曾丹,严璐琪..3个群体方斑东风螺线粒体 COI 基因的遗传多样性分析[J].湖南农业大学学报(自然科学版),2016,42(4):429-434,6.基金项目
海洋公益性行业科研专项经费项目(201205021-1);国家自然科学基金项目(31372530) (201205021-1)