湖南农业大学学报（自然科学版）2016，Vol.42Issue(4)：435-440,6.
富硒黑木耳硒多糖超声-微波提取工艺优化及其抗氧化活性
Process optimization on extracting of selenium polysaccharides with ultrasonic and microwave method from selenium-enriched Auricularia auricula and its antioxidant activity
摘要
Abstract
Selenium polysaccharides extracting from selenium-enriched Auricularia auricula by the ultrasonic and microwave method was used as an index in this test. The effects of different ultrasonic time, microwave time, ultrasonic power, microwave power and ratio of liquor to material on extracting rate of selenium polysaccharides were investigated by single factor experiment, then ultrasonic time, microwave time, microwave power and ratio of liquor to material, the four factors which had significant effects on extraction rate of selenium polysaccharides were chosen to determine the optimum process parameters in response surface test. The results showed that under the conditions of 1∶58 (g/mL) of ratio of liquor to material, 26 min of ultrasonic time, 22 min of microwave time and 350 W of microwave power, the selenium polysaccharides extraction rate and the extraction time were the highest (11.79%) and shortest, which were 4.1% higher and 56.67% shorter than those by the water-extractable method. The results from the antioxidant tests in vitro with different selenium polysaccharides showed that absorbance values of reducing capacity, the IC50 values of scavenging against hydroxyl free radical and the IC50 values of scavenging against DPPH free radical of selenium polysaccharides by the ultrasonic and microwave method and the water-extractable method were 0.431 and 0.410, 3.81 and 4.91 mg/mL, and 4.59 and 5.70 mg/mL, respectively .The antioxidant activity of selenium polysaccharides extracted by ultrasonic and microwave method was better than that of selenium polysaccharides extracted by the water-extractable method.关键词
黑木耳/硒多糖/超声波/微波/抗氧化性Key words
Auricularia auricula/seleniumpolysaccharides/ultrasonic/microwave/antioxidant activity分类
农业科技引用本文复制引用
刘春延,赵博,张国财,郑煜,李建顺,刘丛荣..富硒黑木耳硒多糖超声-微波提取工艺优化及其抗氧化活性[J].湖南农业大学学报（自然科学版）,2016,42(4):435-440,6.基金项目
中央高校基本科研业务费专项资金(2572014AA08)，黑龙江省科学技术厅攻关项目(GB08B202) （2572014AA08）
