海洋科学2016，Vol.40Issue(4)：59-64,6.DOI:10.11759//hykx20140805001
不同磷源对米氏凯伦藻生长和碱性磷酸酶活性的影响
Effects of the different kinds of phosphorus sources on growth and alkaline phosphatase activity (APA) ofKarenia mikimotoi Hansen
摘要
Abstract
To understand the mechanisms of phosphorus nutrition in blooms ofKarenia mikimotoi, the studies of the growth and alkaline phosphatase activity (APA) ofK. mikimotoi were undertaken using different phosphorus sources [adenosine triphosphate disodium salt (adenosine disodium triphosphate, and ATP), glycerophosphate (sodium glyc-erophosphate and G-P), lecithin (lecthin and LEC) and NaH2PO4·2H2O]. The results revealed the following (1)K. miki-motoi could utilize PO4-P, sodium glycerophosphate (G-P), sodium glycerophosphate (G-P), and adenosine triphosphate (ATP) as phosphorus sources, but it could not utilize lecithin (LEC); (2) when the APA ofK. mikimotoiwas assayedat the initial stage, the maximum APA in the different phosphorus sources, ATP, G-P, LEC and NaH2PO4·2H2O, ofK. mikimotoi were 6.0, 10.5, 8.0 and 0.4 pmol/(cell·h), respectively. As the incubation time was increased, the APA decreased and then increased and maintained the maximum level. The phosphorus source of NaH2PO4·2H2O, the APA ofK. mikimotoi did not increase significantly; (3) the APA site of a singleK. mikimotoi cellwas widelydistributed, largely on the cell surface. In this research, it was found thatK. mikimotoi could better absorb and utilize organic phosphorus to maintain growth in the external environment under inorganic phosphorus-limited conditions, which confirmed that the bloom ofK. miki-motoi frequently occurred in phosphorus-restricted areas in recent years.关键词
米氏凯伦藻(Karenia mikimotoi Hansen)/有机磷/碱性磷酸酶Key words
Karenia mikimotoi/organic phosphorus/alkaline phosphatase分类
资源环境引用本文复制引用
庞勇,聂瑞,吕颂辉..不同磷源对米氏凯伦藻生长和碱性磷酸酶活性的影响[J].海洋科学,2016,40(4):59-64,6.基金项目
国家自然科学基金(U0733006) （U0733006）
国家重点基础研究发展计划(973)项目(2010CB428702)[Foundation：National Natural Science Foundation of China, No. U0733006 （973）
National Keystone Basic Research Program(973), No.2010CB428702] （973）
