海洋科学2016，Vol.40Issue(4)：73-80,8.DOI:10.11759/hykx20150509001
响应面法优化鹿角菜中岩藻多糖的提取工艺
Optimization of fucoidan extraction process fromPelvetia siliquosa TsengetC. F. Chang by using response surface methodology
摘要
Abstract
For the development of fucoidan obtained fromPelvetia siliquosaTsenget C. F. Chang (P. siliquosa Tsenget C. F. Chang), fucoidan fromP.siliquosaTsenget C. F. Chang were extracted using the traditional water bath method. Based on a single factor experiment using the Box-Benhnken central composite design method, the process of extracting fucoidan fromP. siliquosaTsenget C. F. Chang was optimized using the response surface method (RSM). Results show that the liquid material ratio, water bath temperature, and water bath time have a sig-nificant effect on the extraction rate of fucoidan. Optimum extraction conditions were as follows: liquid–solid ratio of 32 ml/g, temperature of 90℃, and water bath time of 8.4 h. The extraction rate of fucoidan reached 5.2005%, and compared with the theoretical value of 5.2153% the relative error of the true value was small. The response surface method was found to match well with the actual process, which shows good applicability and feasibility of using the RSM for the optimization of the extraction process of fucoidan fromP. siliquosaTsenget C. F. Chang.关键词
鹿角菜/岩藻多糖/提取/响应面分析Key words
Pelvetia siliquosaTsenget C.F. Chang/Fucoidan/extraction/response surface methodology分类
农业科技引用本文复制引用
宋海燕,何文辉,彭自然,袁荣荣,张泽华..响应面法优化鹿角菜中岩藻多糖的提取工艺[J].海洋科学,2016,40(4):73-80,8.基金项目
上海市科学技术委员会社会发展领域重大科技项目(09DZ120010C) （09DZ120010C）
上海高校水产学一流学科建设项目资助[Fundation：Shanghai major scientific and technological projects in the field of Science and Technology for Social Development Board (09DZ120010C) （09DZ120010C）
Shanghai Colege of Fisheries Science class Academic Discipline Project] （）
