吉林中医药2016，Vol.36Issue(7)：706-708,3.DOI:10.13463/j.cnki.jlzyy.2016.07.017
市售鹿茸饮片及鹿茸粉的 DNA 条形码鉴定
Identification of antler yinpian antler powder components based onDNA barcoding technology
摘要
Abstract
Objective Using the COI sequences of commercially available identification pilose antler,deer antler powder slices,and establish the unified molecular identification method. Methods The different origin of pilose antler yinpian and pilose antler powder respectively for DNA extraction,amplification and sequencing COI sequences,molec-ular identification method,and the commercial antler yinpian,velvet antler powder were investigated. Results All can be used antler yinpian COI universal primers for PCR amplification and sequencing;Distinguish between species is ob-vious;In the sold 40 that 14 pieces are the pharmacopoeia in medicinal species,26 species are the pharmacopoeia reg-ulations. Velvet antler powder successful COI sequences. Conclusion COI sequences of DNA barcode identification method is accurate and effective identification of pilose antler yinpian,this method for market supervision,provide a scientific means of velvet antler slices.关键词
DNA 条形码/COI 序列/鹿茸饮片/鹿茸粉Key words
DNA barcoding technology/pilose antler yinpian/antler powder分类
医药卫生引用本文复制引用
高晓晨,刘冬,崔丽娜,孙佳明,张辉..市售鹿茸饮片及鹿茸粉的 DNA 条形码鉴定[J].吉林中医药,2016,36(7):706-708,3.基金项目
吉林省中医药科技项目（2014- Q19）。 （）
