江苏大学学报（医学版）Issue(4)：292-295,301,5.DOI:10.13312/j.issn.1671-7783.y160076
SPARCL1基因对肝癌 SMMC－7721细胞增殖与凋亡的影响
Influence of SPARCL1 gene on the proliferation and apoptosis of human hepatocellular carcinoma SMMC-7721 cell
摘要
Abstract
Objective: To investigate the influence of secreted protein acidic and rich in cysteines -like 1 (SPARCL1) gene on the proliferation and apoptosis of human hepatocellular carcinoma SMMC -7721 cell. Methods: SMMC-7721 cells were divided into five groups , including blank control group , pIRES2-ZsGreen vector group, pIRES2-ZsGreen-SPARCL1 group, negative siRNA group and SPARCL1 siRNA group.West-ern blotting was used to investigate SPARCL1 expression, while fluorescence microscope were used to verify the transfection efficiency of SPARCL1.MTT assay was used to detect cell proliferation ability ; and flow cy-tometry was used to measure apoptosis rate .Results: The transfection efficiency of SPARCL1 was high through the fluorescence microscope observation and western blotting .SPARCL1 expression, the cell inhibi-tion ratio, and the apoptosis ratio in pIRES2-ZsGreen-SPARCL1 group was significantly higher than other four groups(P <0.05), while SPARCL1 siRNA group was lower than other four groups (P <0.05).Con-clusion: SPARCL1 overexpression elevated the proliferation inhibition ratio and apoptosis ratio of human hepatocellular carcinoma SMMC-7721 cell; on the contrary, SPARCL1 silencing lowered.关键词
SPARCL1/肝细胞癌/细胞增殖/细胞凋亡Key words
SPARCL1/hepatocellular carcinoma/cell proliferation/apoptosis分类
医药卫生引用本文复制引用
韩玮,徐旋旋,何大伟,曹方,丁厚中..SPARCL1基因对肝癌 SMMC－7721细胞增殖与凋亡的影响[J].江苏大学学报（医学版）,2016,(4):292-295,301,5.基金项目
昆山市科技计划资助项目 （）
