江苏大学学报（医学版）Issue(4)：311-315,5.DOI:10.13312/j.issn.1671-7783.y160026
透明质酸功能化三氧化二钆纳米颗粒的制备及其放疗增敏作用
Hyaluronic acid-functionalized gadolinium oxide nanoparticles for radiosensitization enhancements
摘要
Abstract
Objective: To investigate the radiosensitization of a newly developed nanopartical modified by hyaluronic acid(HA-Gd2 O3 ) in hepatoma HepG-2 cells.Methods:We used HA and GdCl 3 as precur-sors to synthesize HA-Gd2 O3 by polyol method.HA-Gd2 O3 were characterized by transmission electron mi -croscopy and X-ray diffraction.Inhibition rates of cell proliferation with different concentrations of HA -Gd2 O3 (0 -200 g/mL) at 24 h after treatment were determined by CCK -8 assay.The tissue compatibility of HA-Gd2 O3 was evaluated by histological analysis .The cells were randomly divided to four groups (control, HA-Gd2 O3 , radiation and HA-Gd2 O3 +radiation), and CCK-8 assay and clonogenic assay were used to e-valuate radiosensitization.Results:We fabricated the HA-Gd2 O3 with a diameter of 112 nm and the amor-phous phase structure.Inhibition rates of cell proliferation of HA -Gd2 O3 ranging from 0, 25, 50, 100, 200 mg/L were 0%, (0.640 ±0.024)%, (3.943 ±0.063)%, (4.871 ±0.062)%, and (4.084 ± 0.076)% with no significant differences(P >0.05).HA-Gd2 O3 was no obvious toxicity to the mouse sus-ceptible organs (liver, heart, spleen, lung, and kidney) showed by histological assessment .Compared with control group, radiation group and HA-Gd2 O3 group, HA-Gd2 O3 +radiation group showed lower inhi -bition rates of cell proliferation and survival fraction (P <0.05).Conclusion:HA-Gd2 O3 was successfully developed, with favorable solubility in water, excellent biocompatibility, no obvious toxicity and effective radiosensitization in hepatoma HepG-2 cells.关键词
三氧化二钆/放疗增敏/纳米材料/透明质酸/HepG-2 细胞Key words
HA-Gd2 O3/radiosensitization enhancement/nanoparticles/hyaluronic acid/HepG-2 cell分类
医药卫生引用本文复制引用
张丽,娄嘉明,王燕,金洁,龚爱华,杜凤移,吴朝阳..透明质酸功能化三氧化二钆纳米颗粒的制备及其放疗增敏作用[J].江苏大学学报（医学版）,2016,(4):311-315,5.基金项目
镇江市社会发展项目 （）
