吉首大学学报（自然科学版）2016，Vol.37Issue(4)：29-34,6.DOI:10.3969/j.cnki.jdxb.2016.04.007
短时低温对绞股蓝种子萌发的促进作用
Effects of Short-Term Low Temperature on the Germination of Gynostemma Pentaphyllum Seeds
摘要
Abstract
In order to explore the method of improving the germination rate of Gynostemma pentaphyllum seeds ,we investigated the effects of different factors ,namely ,illumination and temperature ,hormone , storage ,low temperature stratification and short‐term low temperature .The results showed that the opti‐mal germination temperature ranged from 15℃ to 25 ℃ ;illumination raised the germination rate ,with the highest germination rate(31 .54 ± 2 .04)% at the optimum temperature of 20 ℃ .It was found that Cytokinins ,such as 6 BA and zeatin ,had no effect on the promotion of the germination ,and Gibberellin , like GA3 ,had almost no impact ,but GA4 promoted the germination to (54 .00 ± 2 .65)% .At 18 ℃ ,Gy‐nostemma pentaphyllum seeds were stored for 30 ,90 ,and 180 days .The results showed that appropriate storage time was conducive to break the dormancy mechanism of Gynostemma pentaphyllum seed ,but when the storage time was too long ,the germination rate declined rapidly ,or even to zero .The statistics of low temperature stratification processing showed that the effect of low temperature stratification was not obvious .Under low temperature stratification for 90 days ,the highest germination rate was only (34 . 26 ± 3 .21)% .By short‐term low temperature treatment ,it was found that short‐term low temperature (9 days of moist storage at 5 ,10 ,15 ℃) increased the germination rate to (73 .06 ± 2 .12)% ,(83 .14 ± 3 . 25)% and (72 .13 ± 3 .52)% .Comparison of storage treatment and post -storage short‐term low temper‐ature treatment showed that ,with the increase in storage time ,the germination rate decreased rapidly , w hile short‐term low temperature treatment still promoted the germination of long -stored seeds .关键词
绞股蓝/萌发率/低温层积/光照/存储时间/植物激素Key words
Gynostemma pentaphyllum/germination rate/low temperature stratification/illumination/short-term low temperature/storage time/plant hormone分类
农业科技引用本文复制引用
吴小玉,彭小列,刘世彪,李晓明,田向荣..短时低温对绞股蓝种子萌发的促进作用[J].吉首大学学报（自然科学版）,2016,37(4):29-34,6.基金项目
国家自然科学基金资助项目（31260039，31360062）；湖南省教育厅高等学校研究项目（09B082）；湖南省重点学科建设项目（JS U 0713Z ）；湖南省高校科技创新团队项目 （）
