军事医学2016，Vol.40Issue(7)：541-544,4.DOI:10.7644/j.issn.1674-9960.2016.07.002
PM2．5通过诱导AP-1活化介导支气管上皮细胞中VEGF的诱导表达和炎症反应
PM2.5 induces VEGF expression and inflammatory responses by trans-activating AP-1 in human bronchial epithelial cells
摘要
Abstract
Objective To explore the role of the transcriptional factor activator protein (AP)-1 in mediating vascular endothelial growth factor ( VEGF) expression in human bronchial epithelial cells exposed to PM 2.5.Methods Beas-2B cells was treated with PM2.5.Luciferase assay was used to detect the activation status of AP-1 and transcription of VEGF in the Beas-2B cells.The induced activation of c-Jun, ATF2 and VEGF expression was tested by Western blotting assay.Results PM2.5 induced transactivation of the transcriptional factor AP-1, accompanied by phosphorylation of the AP-1 components, c-Jun and ATF2 in Beas-2B cells.Moreover, when AP-1 activation was inhibited by knocking down c-Jun or ATF2 expressions, induction of VEGF expression was partially attenuated in Beas-2B cells.Conclusion AP-1 is a critical transcriptional factor in mediating PM2.5-induced VEGF expression and inflammatory responses in human bronchial epithelial cells.关键词
细颗粒物/转录因子激活蛋白1/血管内皮生长因子类/人支气管上皮细胞/炎症Key words
particulate matter 2.5/transcription factors activator protein 1/vascular endothelial growth factors/human bronchial epithelial cells/inflammation分类
医药卫生引用本文复制引用
徐秀段,敖登其木格,刘莎莎,王红丽,胡永亮,胡美茹,宋伦..PM2．5通过诱导AP-1活化介导支气管上皮细胞中VEGF的诱导表达和炎症反应[J].军事医学,2016,40(7):541-544,4.基金项目
国家973计划资助项目 （）
